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细胞核中DNA-醛类物质染色的最新进展

Recent progress in the staining of DNA-aldehyde in cell nuclei.

作者信息

Dutt M K

出版信息

Acta Histochem. 1976;56(1):120-39. doi: 10.1016/S0065-1281(76)80034-7.

Abstract

The paper is a review of studies on Feulgen staining of nuclear DNA. For convenience, the paper is split into several sections, such as (a) the characteristic of the dyes used for the manufacture of Schiff reagent (b) preparations of Schiff reagent (c) fixation of the material (d) hydrolysis (e) staining with Schiff reagent (f) Schiff-type dyes and (g) use of Schiff-type dyes in quantitative cytochemistry. Barring the section on preparation of Schiff reagent that includes the classical methods, all other sections contain a comprehenside account showing progress made during the last 15 years. Under each section interpretations of new findings as far as possible, and in the section on staining with Schiff reagent, new approaches to its study have been presented. The section on Schiff-type dyes includes dyes with and without primary amino group.

摘要

本文是对核DNA福尔根染色研究的综述。为方便起见,本文分为几个部分,例如:(a)用于制备席夫试剂的染料特性;(b)席夫试剂的制备;(c)材料固定;(d)水解;(e)用席夫试剂染色;(f)席夫型染料;(g)席夫型染料在定量细胞化学中的应用。除了包含经典方法的席夫试剂制备部分外,所有其他部分都全面介绍了过去15年取得的进展。在每个部分尽可能对新发现进行了解释,在席夫试剂染色部分,还介绍了其研究的新方法。席夫型染料部分包括含和不含伯氨基的染料。

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