Bert J L, Pearce R H, Mathieson J M, Warner S J
Biochem J. 1980 Dec 1;191(3):761-8. doi: 10.1042/bj1910761.
The volumes from which 3H-labelled dextrans are excluded by dermal collagenous fibres were calculated by dilution of dextran probes. Five dextrans, of average Stokes' radii 1.72, 2.53, 3.92, 4.54 and 14.24nm, were investigated at concentrations between 0.1 and 3% (w/w). The excluded volume was dependent on dextran concentration only for the two smaller probes. The largest dextran was shown not to bind to the fibres. A plot of the square root of excluded volume against Stokes' radius was linear for the four smallest dextrans, corresponding to the predictions of Ogston's [(1958) Trans. Faraday Soc. 54, 1754--1757] rod-and-sphere model of fibrous exclusion, and suggesting that dextrans of Stokes' radius between 1.72 and 4.54 nm were excluded by a cylindrical solid fibre of radius 2.90 +/- 0.72 nm. Larger molecules were excluded by a structure of much greater size, since the volume exclusion for the largest dextran was only slightly greater than that of the dextran less than one-third its radius. The excluded volume of 3H2O fell slightly below the line describing the dextran data, indicating that water had access to most of the volume not occupied by the collagenous fibres.
通过葡聚糖探针的稀释来计算被真皮胶原纤维排斥的³H标记葡聚糖的体积。研究了五种平均斯托克斯半径分别为1.72、2.53、3.92、4.54和14.24nm的葡聚糖，浓度范围在0.1%至3%（w/w）之间。仅对于两种较小的探针，排斥体积取决于葡聚糖浓度。最大的葡聚糖未显示与纤维结合。对于四种最小的葡聚糖，排斥体积的平方根与斯托克斯半径的关系图呈线性，这与奥格斯顿[(1958年)《法拉第学会会刊》54, 1754 - 1757]的纤维排斥棒球模型的预测相符，表明斯托克斯半径在1.72至4.54nm之间的葡聚糖被半径为2.90±0.72nm的圆柱形实心纤维排斥。较大的分子被尺寸大得多的结构排斥，因为最大葡聚糖所占据的排斥体积仅略大于其半径不到三分之一的葡聚糖的排斥体积。³H₂O的排斥体积略低于描述葡聚糖数据的直线，表明水能够进入大部分未被胶原纤维占据的体积。
