Wiederhielm C A, Black L L
Am J Physiol. 1976 Aug;231(2):638-41. doi: 10.1152/ajplegacy.1976.231.2.638.
The osmotic interaction of plasma proteins with collagen and hyaluronate has been evaluated by measuring the oncotic pressure of mixed solutions of varying composition. Collagen, despite its insolubility, exhibits a pronounced volume exclusion effect on plasma proteins, and the oncotic pressure of mixed solutions is considerably higher than that of the plasma protein stock solution. The volume exclusion of collagen on small molecules such as sucrose is negligible. A solution composed of 1.6% plasma proteins, 20% collagen, and .4% hyaluronate in Ringer solution, approximating the composition of the interstitium, was found to yield higher oncotic pressures than those previously reported from the interstitium. The probable role of impurities and degradation in the isolation process is discussed. Results reported earlier from in vitro and in vivo studies indicated that tissue oncotic pressures are considerably higher than generally recognized and that tissue fluid is in probable osmotic equilibrium with lymph in skin and muscle.
通过测量不同组成混合溶液的胶体渗透压，评估了血浆蛋白与胶原蛋白和透明质酸盐之间的渗透相互作用。胶原蛋白尽管不溶，但对血浆蛋白表现出明显的体积排阻效应，混合溶液的胶体渗透压远高于血浆蛋白储备溶液。胶原蛋白对蔗糖等小分子的体积排阻可忽略不计。发现在林格氏溶液中由1.6%血浆蛋白、20%胶原蛋白和0.4%透明质酸盐组成的溶液，其成分接近间质，产生的胶体渗透压高于先前报道的间质渗透压。讨论了分离过程中杂质和降解的可能作用。早期体外和体内研究报告的结果表明，组织胶体渗透压远高于普遍认知，并且组织液可能与皮肤和肌肉中的淋巴处于渗透平衡。
