Fujiwara K, Yasuno M, Kitagawa T
Cancer Res. 1981 Oct;41(10):4121-6.
An antibody directed toward pepleomycin, a new antitumor antibiotic related structurally to bleomycin, has been produced in rabbits by immunization with a pepleomycin-protein conjugate which was prepared by a novel procedure of coupling pepleomycin to mercaptosuccinylated bovine serum albumin using N-(gamma-maleimidobutyryloxy)succinimide as a coupling agent. The antiserum was monospecific to pepleomycin and showed almost no cross-reactivity with a variety of other bleomycin analogs. An enzyme immunoassay for pepleomycin has been developed utilizing this antiserum and beta-D-galactosidase-labeled pepleomycin. The lower limit of detection by this assay, which involves a double antibody technique for the separation of antibody-bound and free antigen, was 50 pg of pepleomycin per tube. Using this assay, drug levels were easily determined in blood and urine of rabbits following administration of pepleomycin in a single dose of 1.2 mg/kg i.v. This assay is also suitable for measuring pepleomycin in the presence of other drugs since the assay is not significantly affected by any other antineoplastic agents tested. Since pepleomycin is now undergoing clinical trial, the enzyme immunoassay of the drug will be a valuable tool in clinical pharmacological studies.
通过用一种新霉素 - 蛋白质偶联物免疫家兔，制备出了一种针对新霉素的抗体。新霉素是一种结构上与博来霉素相关的新型抗肿瘤抗生素，该偶联物是通过一种新的方法制备的，即使用N -（γ - 马来酰亚胺丁酰氧基）琥珀酰亚胺作为偶联剂，将新霉素与巯基琥珀酰化牛血清白蛋白偶联。抗血清对新霉素具有单特异性，与多种其他博来霉素类似物几乎没有交叉反应。利用这种抗血清和β - D - 半乳糖苷酶标记的新霉素，开发了一种新霉素的酶免疫测定法。该测定法采用双抗体技术分离抗体结合的和游离的抗原，每管检测下限为50 pg新霉素。使用该测定法，在以1.2 mg/kg静脉注射单剂量新霉素后，可轻松测定家兔血液和尿液中的药物水平。该测定法也适用于在其他药物存在的情况下测定新霉素，因为所测试的任何其他抗肿瘤药物对该测定法均无显著影响。由于新霉素目前正在进行临床试验，该药物的酶免疫测定法将成为临床药理学研究中的一种有价值的工具。
