Aherne G W, Saesow N, James S, Marks V
Cancer Treat Rep. 1982 Jun;66(6):1365-70.
A rapid sensitive radioimmunoassay for pepleomycin (PM) has been developed using an antiserum produced in a sheep in response to a bovine serum albumin-drug conjugate. Radioiodinated PM was prepared using Iodogen. The assay was reproducible with a limit of detection in plasma of 200 ng/liter. The antiserum does not cross-react with bleomycin or with a number of unrelated drugs. Pharmacokinetic studies using the assay were carried out in three patients who received a short iv infusion of 5 mg/m2 of PM. The drug disappeared from the plasma with an elimination half-life of approximately 3 hours.
已开发出一种用于平阳霉素（PM）的快速灵敏放射免疫分析法，该方法使用绵羊针对牛血清白蛋白 - 药物偶联物产生的抗血清。使用碘代甘氨酸制备放射性碘化平阳霉素。该分析方法可重复，血浆检测限为200纳克/升。该抗血清与博来霉素或多种无关药物无交叉反应。使用该分析方法对三名接受5毫克/平方米平阳霉素短时间静脉输注的患者进行了药代动力学研究。药物从血浆中消失，消除半衰期约为3小时。
