Sun T, Lam K W, Narukar L
Clin Chem. 1981 Oct;27(10):1742-4.
We compared electrophoretic evaluation of acid phosphatase isoenzymes with spectrophotometric determination of prostatic acid phosphatase in terms of clinical utility. In all of 33 cases of prostatic carcinoma, an increased prostatic fraction was detected; in nine prostatectomized patients, this fraction returned to normal as measured by either technique. Abnormal spectrophotometric results were also seen in 10 cases of benign prostatic hypertrophy and seven cases of non-prostatic disorders, but only two benign prostatic hypertrophy and one non-prostatic case showed a prostatic band (band 2) in an electrophoretogram. Band 2 was not demonstrated in 463 patients affected by a great variety of diseases but without prostatic disorders. A weak band 5 was seen in patterns for most patients, except for cases with metastatic bone tumor and Gaucher's disease, whose serum showed a strong band 5. The specificity of bands 2 and 5 seems to be confirmed by this large series of patients. Measurement of acid phosphatase isoenzymes is recommended as a routine screening test for patients whose serum acid phosphatase is abnormally high, because the isoenzyme study not only indicates the presence or absence of prostatic cancer but also whether or not there is bony metastasis. Other disorders such as Gaucher's disease, different kinds of leukemias, and thrombocythemia may also be detected and distinguished by this screening technique.
我们从临床实用性方面比较了酸性磷酸酶同工酶的电泳评估与前列腺酸性磷酸酶的分光光度测定。在33例前列腺癌患者中，均检测到前列腺部分升高；在9例接受前列腺切除术的患者中，通过任何一种技术测量，该部分均恢复正常。在10例良性前列腺增生和7例非前列腺疾病患者中也出现了异常的分光光度测定结果，但只有2例良性前列腺增生和1例非前列腺疾病患者在电泳图中显示出前列腺带（带2）。在463例患有各种疾病但无前列腺疾病的患者中未显示带2。除了转移性骨肿瘤和戈谢病患者血清显示强带5外，大多数患者的图谱中可见弱带5。这一大系列患者似乎证实了带2和带5的特异性。对于血清酸性磷酸酶异常升高的患者，建议将酸性磷酸酶同工酶测定作为常规筛查试验，因为同工酶研究不仅可以表明前列腺癌的存在与否，还可以表明是否存在骨转移。这种筛查技术还可以检测和区分其他疾病，如戈谢病、不同类型的白血病和血小板增多症。
