戈谢病中的酸性磷酸酶同工酶

Acid phosphatase isoenzymes in Gaucher's disease.

作者信息

Mercer D W, Peters S P, Glew R H, Lee R E, Wenger D M

出版信息

Clin Chem. 1977;23(4):631-5.

Abstract

Acid phosphatase (EC 3.1.3.2) isoenzyme profiles of extracts of splenic tissue and serum from patients with Gaucher's disease were measured by a mini-column ion-exchange chromatographic method [Clin. Chem., 23, 000 (1977)]. Diagnosis of Gaucher's disease in the five patients studied was confirmed by demonstrating decreased (2.3 to 4.1% of normal) glucocerebrosidase activity in the spleen. With p-nitrophenyl phosphate as substrate, increased acid phosphatase activity (three-to eight-fold normal) was demonstrated in spleen tissue from Gaucher;s disease patients; isoenzyme profiles by the ion-exchange column technique showed acid phosphatase isoenzyme 5 to be the predominant isoenzyme. Comparison of acid phosphatase isoenzyme profiles from patients with Gaucher's disease and prostatic carcinoma revealed distinct differences in the activities of isoenzymes 2 and 5. The isoenzyme-5 measurement thus appears to provide a diagnostic test for Gaucher's disease that can be done reapidly and easily in the routine clinical chemistry laboratory.

摘要

采用微型柱离子交换色谱法[《临床化学》，23, 000 (1977)]测定了戈谢病患者脾脏组织提取物和血清中的酸性磷酸酶（EC 3.1.3.2）同工酶谱。通过证明所研究的5例患者脾脏中葡萄糖脑苷脂酶活性降低（为正常水平的2.3%至4.1%），确诊为戈谢病。以对硝基苯磷酸为底物，发现戈谢病患者脾脏组织中的酸性磷酸酶活性增加（为正常水平的三至八倍）；离子交换柱技术测定的同工酶谱显示酸性磷酸酶同工酶5是主要的同工酶。比较戈谢病患者和前列腺癌患者的酸性磷酸酶同工酶谱发现，同工酶2和5的活性存在明显差异。因此，同工酶5的测定似乎为戈谢病提供了一种诊断试验，可在常规临床化学实验室快速、简便地进行。