Kawamura H, Hashimoto Y
Gan. 1980 Aug;71(4):507-13.
Cellular retinol-binding protein (CRBP) in cancers and in vitro-transformed epithelial cells of the ACI/N rat urinary bladder was assayed with radiolabeled retinol by sucrose density gradient centrifugation. CRBP was detected in abundance in tumor tissues of all 5 transplantation bladder cancer lines. However, this protein was below the limits of detection in bladder cancer cells of culture lines that had originated from the same primary cancers as the corresponding transplantation lines. CRBP was detected in small quantity in in vitro-transformed epithelial cells of a culture line but it was absent in another line of similar origin. The addition of retinol to the culture medium prevented the in vitro keratinization of CRBP-positive cells but it had no effect on the keratinization of CRBP-negative cells.
通过蔗糖密度梯度离心法，用放射性标记的视黄醇对ACI/N大鼠膀胱的癌症组织和体外转化的上皮细胞中的细胞视黄醇结合蛋白（CRBP）进行了检测。在所有5种移植性膀胱癌系的肿瘤组织中均大量检测到CRBP。然而，在与相应移植系起源于同一原发性癌症的培养系膀胱癌细胞中，这种蛋白质低于检测限。在一个培养系的体外转化上皮细胞中少量检测到CRBP，但在另一个起源相似的细胞系中未检测到。向培养基中添加视黄醇可阻止CRBP阳性细胞的体外角质化，但对CRBP阴性细胞的角质化没有影响。
