Kawamura H, Hashimoto Y
Gan. 1979 Aug;70(4):483-91.
In vitro and in vivo transformed epithelial cells of the rat urinary bladder keratinized well on a coverslip culture. Keratinization proceeded more rapidly in in vitro-transformed cells than in in vivo-induced bladder cancer cells. The grade of keratinization was estimated from the absorption spectrum of a papanicolaou-stained specimen which afforded two peaks derived from keratinized cells and viable cells. Using this semiquantitative method, effect of various drugs on keratinization was studied on 3 lines of in vitro-transformed epithelial cells. Vitamin A and its analogs prevented the keratinization in 2 out of 3 lines at dosage over 1 micrograms/ml, but other drugs such as vitamin C, E, and K, steroid hormones, cyclic AMP, polyamines, polyanions, and dimethyl sulfoxide were ineffective for preventing keratinization. Amino acid compositions of keratinized cells and viable cells were not fundamentally different.
大鼠膀胱的体外和体内转化上皮细胞在盖玻片培养上角质化良好。体外转化细胞的角质化比体内诱导的膀胱癌细胞进行得更快。根据巴氏染色标本的吸收光谱估计角质化程度，该光谱给出了来自角质化细胞和活细胞的两个峰值。使用这种半定量方法，研究了各种药物对3株体外转化上皮细胞角质化的影响。维生素A及其类似物在剂量超过1微克/毫升时可阻止3株细胞中的2株发生角质化，但其他药物如维生素C、E和K、类固醇激素、环磷酸腺苷、多胺、聚阴离子和二甲亚砜在防止角质化方面无效。角质化细胞和活细胞的氨基酸组成没有根本差异。
