Glebov O K, Polianskaia G G, Pinaev G P
Tsitologiia. 1981 Sep;23(9):1041-6.
The metaphase chromosomes (MC) isolated from the Chinese hamster cells were identified with the aid of differential staining (G-bands). It was shown that differences in the relative recognizability of MC in metaphase plates and after their isolation are determined by changes in composition of isolated MC, rather than by those in staining capacity of MC after their isolation. The frequencies of identified MC are constant and independent upon the type of MC preparations and relation between identified and unidentified MC in certain preparations. At allows to apply the described method for the analysis of chromosome fractionation, using changes in frequencies of identified MC as a criterion of efficiency of the fractionation method. Possible ways of increasing the recognizability level of isolated MC are discussed.
借助鉴别染色（G带）对从中国仓鼠细胞中分离出的中期染色体（MC）进行了鉴定。结果表明，中期板中MC与其分离后的相对可识别性差异是由分离出的MC组成变化决定的，而非由其分离后染色能力的变化决定。已鉴定MC的频率是恒定的，且与MC制备类型以及特定制备中已鉴定和未鉴定MC之间的关系无关。这使得可以将所描述的方法应用于染色体分级分析，将已鉴定MC频率的变化作为分级方法效率的标准。文中还讨论了提高分离出的MC可识别性水平的可能方法。
