Rodionov V M, Kuz'micheva N A, Pospelova A V, Pleskova M V
Vopr Med Khim. 1981;27(4):569-72.
An attempt was made to isolate the liver tissue chalone blocking the transition of hepatocytes from G2 period of the cell cycle into mitosis/g2-chalone/. The purification was carried out using gel filtration through Sephadex G-75 and hydroxyapatite chromatography as well as by means of ultrafiltration through Diaflo membranes XM-300, XM-100 and PM-10. The biologically active preparation, obtained by these two procedures, was purified 100-fold. Molecular mass of G2 chalone was apparently less then 10,000; it penetrated through PM-10 membrane. The chalone appears to form complexes with high molecular carriers.
曾尝试分离阻止肝细胞从细胞周期的G2期进入有丝分裂的肝脏组织抑素（G2-抑素）。通过Sephadex G-75凝胶过滤、羟基磷灰石色谱法以及使用Diaflo XM-300、XM-100和PM-10膜进行超滤来进行纯化。通过这两种方法获得的生物活性制剂纯化了100倍。G2抑素的分子量明显小于10,000；它能透过PM-10膜。该抑素似乎与高分子载体形成复合物。
