Sokolova T M, Kisling W, Khil'ko S N, Fel'gengaueér P E, Tazulakhova E B
Vopr Virusol. 1981 May-Jun(3):339-47.
A further study of conditions for induction, determinations of activity and products of mRNAs translation of AVP having nonspecific antiviral activity from L-929 cells treated with poly(I) . poly(C) was carried out. Under conditions of superinduction of interferon the yield of AVP mRNA is reduced unlike that of interferon mRNA. Manifestations of the antiviral effect of AVP mRNA translation products do not seem to require the stages of transcription as dihydrorifampicin exerts no influence on this process. The inhibiting effect of actinomycin D is more likely to be associated with its effect on the energy of cells and synthesis of macroergic compounds of ATP type. Active AVP mRNAs from L-929 cells like interferon mRNA consist mainly of poly-A-deficient mRNAs. The sedimentation rate of AVP mRNAs differs from that of interferon mRNA and is about 10S. AVP mRNA translation products in ovocytes of Xenopus laevis, in contrast to control mRNAs, contain isoadenylate synthetase responsible for synthesis of 2'5'-oligoisoadenylate, a nonspecific translation inhibitor the mechanism of action of which consists in activation of cellular endogenous nuclease. The results suggest that the mode of development of the antiviral condition by activation of isoadenylate synthetase is similar in the cells treated with interferon and poly(I) . poly(C).
对用聚肌苷酸-聚胞苷酸（poly(I).poly(C)）处理的L-929细胞中具有非特异性抗病毒活性的精氨酸加压素（AVP）的诱导条件、活性测定以及mRNA翻译产物进行了进一步研究。在干扰素超诱导条件下，AVP mRNA的产量与干扰素mRNA不同，会降低。AVP mRNA翻译产物的抗病毒作用表现似乎不需要转录阶段，因为二氢利福平对此过程没有影响。放线菌素D的抑制作用更可能与其对细胞能量和ATP类型高能化合物合成的影响有关。L-929细胞中的活性AVP mRNA与干扰素mRNA一样，主要由缺乏多聚腺苷酸的mRNA组成。AVP mRNA的沉降速率与干扰素mRNA不同，约为10S。与对照mRNA相比，非洲爪蟾卵母细胞中AVP mRNA的翻译产物含有负责合成2',5'-寡聚异腺苷酸的异腺苷酸合成酶，这是一种非特异性翻译抑制剂，其作用机制在于激活细胞内源性核酸酶。结果表明，在用干扰素和聚肌苷酸-聚胞苷酸（poly(I).poly(C)）处理的细胞中，通过激活异腺苷酸合成酶产生抗病毒状态的方式是相似的。
