Scholz K U
Cytometry. 1981 Nov;2(3):159-64. doi: 10.1002/cyto.990020305.
A general procedure was developed to evaluate two-parameter flow cytometric data. These data usually show overlapping distributions representing different subpopulations. To calculate the fractions of cells in the various subpopulations, the maximum likelihood method is used, assuming an unknown number of Gaussian distributions with constant coefficient of variation in the histogram. A test on simulated data shows that Gaussians can be separated clearly by the procedure if the distance between their mean values is larger than twice the maximum standard deviation. The analysis of different measurements of double-stained (DNA/protein) cultured cells out of the same pool yield the same fractions in the phases of the cell cycle with acceptable error rates. To simulate samples with different fractions in the phases of the cell cycle, defined mixtures of proliferating and resting cells of the same type were prepared and measured for DNA and cell size. The calculated fractions are consistent with the expected variation of G1, S and G2 + M fractions depending on the mixture ratio.
开发了一种通用程序来评估双参数流式细胞术数据。这些数据通常显示代表不同亚群的重叠分布。为了计算各个亚群中的细胞比例，使用最大似然法，假设直方图中高斯分布的数量未知且变异系数恒定。对模拟数据的测试表明，如果高斯分布的均值之间的距离大于最大标准差的两倍，则可以通过该程序清楚地分离它们。对来自同一细胞池的双染（DNA/蛋白质）培养细胞的不同测量进行分析，在细胞周期各阶段得到的比例相同，误差率可接受。为了模拟细胞周期各阶段具有不同比例的样本，制备了相同类型的增殖细胞和静止细胞的定义混合物，并测量其DNA和细胞大小。计算得到的比例与根据混合比预期的G1、S和G2+M比例变化一致。
