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二氨基联苯胺染色后通过显微分光光度法对过氧化氢酶活性进行定量分析。

Quantitation of catalase activity by microspectrophotometry after diaminobenzidine staining.

作者信息

Geerts A, Roels F

出版信息

Histochemistry. 1981;72(3):357-67. doi: 10.1007/BF00501778.

DOI:10.1007/BF00501778
PMID:6170616
Abstract

The absorbance of the reaction product of catalase staining with diaminobenzidine is linearly proportional to enzyme activity. This is shown in semithin Epon sections of model systems containing serum albumin and catalase from bovine or guinea pig liver. Absorbance measurements were also performed on semithin sections of guinea pig liver, and from these, the activity of cytoplasmic (extraperoxisomal) catalase has been derived.

摘要

过氧化氢酶与二氨基联苯胺染色反应产物的吸光度与酶活性呈线性比例关系。在含有牛血清白蛋白和来自牛或豚鼠肝脏过氧化氢酶的模型系统的半薄环氧树脂切片中可以看出这一点。还对豚鼠肝脏的半薄切片进行了吸光度测量，并由此得出了细胞质（过氧化物酶体外）过氧化氢酶的活性。

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1
Quantitation of catalase activity by microspectrophotometry after diaminobenzidine staining.二氨基联苯胺染色后通过显微分光光度法对过氧化氢酶活性进行定量分析。
Histochemistry. 1981;72(3):357-67. doi: 10.1007/BF00501778.
2
An assessment of the DAB methods for cytochemical detection of catalase and peroxidase.用于过氧化氢酶和过氧化物酶细胞化学检测的二氨基联苯胺（DAB）方法的评估
J Histochem Cytochem. 1979 Oct;27(10):1365-6. doi: 10.1177/27.10.92493.
3
Inhibition of catalase by 3,3'-diaminobenzidine.3,3'-二氨基联苯胺对过氧化氢酶的抑制作用。
Biochem J. 1985 Mar 15;226(3):781-7. doi: 10.1042/bj2260781.
4
Microspectrophotometric quantitation of the diaminobenzidine reaction for histochemical demonstration of cytochrome oxidase activity.用于细胞色素氧化酶活性组织化学显示的二氨基联苯胺反应的显微分光光度法定量分析。
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Immunoperoxidase methods: increased efficiency using fluorescence microscopy for 3,3-diaminobenzidine (DAB) stained semithin sections.免疫过氧化物酶方法：使用荧光显微镜提高3,3-二氨基联苯胺（DAB）染色半薄切片的效率。
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Selective staining of cell organelles in rat liver with 3,3'-diaminobenzidine.用3,3'-二氨基联苯胺对大鼠肝脏中的细胞器进行选择性染色。
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Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH.咪唑可提高在中性pH条件下用二氨基联苯胺进行过氧化物酶细胞化学反应的灵敏度。
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New data concerning the nucleolar DNA organization in two cellular types after electronic opacification of the nucleic acid containing structures by oxidized diaminobenzidine.
Acta Histochem Suppl. 1979;20:79-93.
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本文引用的文献

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The distributional error in microspectrophotometry.显微分光光度法中的分布误差。
Lab Invest. 1952;1(2):250-65.
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Sensitivity and efficiency of four immunohistochemical methods as defined by staining of artificial sections.通过人工切片染色定义的四种免疫组织化学方法的敏感性和效率。
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Pt-staining of peroxidatic reaction products at the ultrastructural level.在超微结构水平上对过氧化物反应产物进行铂染色。
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Matrix models. Essential tools for microscopic cytochemical research.矩阵模型。微观细胞化学研究的重要工具。
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Some aspects of the regulation and transport in enzyme membrane models.酶膜模型中的调节与转运的某些方面。
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6
Electron microscopic and kinetic studies dealing with an artificial enzyme membrane. Application to a cytochemical model with the horseradish peroxidase-3,3'-diaminobenzidine system.关于人工酶膜的电子显微镜和动力学研究。应用于辣根过氧化物酶-3,3'-二氨基联苯胺系统的细胞化学模型。
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Cytomorphometry of developing rat liver and its application to enzymic differentiation.发育中大鼠肝脏的细胞形态计量学及其在酶分化中的应用。
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Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue.组织分级分离研究。17. 大鼠肝脏组织中单胺氧化酶、天冬氨酸转氨酶、丙氨酸转氨酶、D-氨基酸氧化酶和过氧化氢酶的细胞内分布。
Biochem J. 1964 Jul;92(1):179-84. doi: 10.1042/bj0920179.
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The effect of glutaraldehyde on catalase. Biochemical and cytochemical studies with beef liver catalase and rat liver peroxisomes.戊二醛对过氧化氢酶的影响。对牛肝过氧化氢酶和大鼠肝过氧化物酶体的生化及细胞化学研究。
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[Cytochemical distinction between catalase and peroxidase].[过氧化氢酶与过氧化物酶的细胞化学鉴别]
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