Mitsuzawa E, Yasuda T, Tamura N, Ohtani S
J Neurol Sci. 1981 Oct;52(1):133-45. doi: 10.1016/0022-510x(81)90141-6.
Experimental allergic encephalomyelitis (EAE) was induced in guinea pigs with bovine myelin basic protein (BP) with adjuvant of either synthetic muramyl dipeptide (Mdp) or Mycobacterium tuberculosis (Tbc). The following results were obtained: (1) The body temperature of the animals was studied serially after sensitization and its elevation was shown to be an early sign of EAE. (2) Several animals developed the clinical and histological signs of hyperacute EAE. (3) An optimal combined dosage of BP and adjuvant was found for induction of clinical EAE and for the the production of complement fixing (CF) antibodies. (4) Little passive hemagglutinating (PH) antibody was produced by single immunization. These results displayed no essential difference in EAE induced by either adjuvant. (5) Detectable PH antibodies developed later in addition to CF antibodies in a few animals immunized with Tbc adjuvant. These animals were skin-tested to BP, and had recovered from body weight loss or limb weakness. The results suggest that humoral antibodies play a role in modifying the disease process, even if they are not essential in production of EAE.
用牛髓磷脂碱性蛋白（BP）与合成的胞壁酰二肽（Mdp）或结核分枝杆菌（Tbc）佐剂在豚鼠中诱发实验性变应性脑脊髓炎（EAE）。获得了以下结果：（1）致敏后连续研究动物体温，其升高被证明是EAE的早期体征。（2）几只动物出现了超急性EAE的临床和组织学体征。（3）发现了用于诱导临床EAE和产生补体结合（CF）抗体的BP和佐剂的最佳联合剂量。（4）单次免疫产生的被动血凝（PH）抗体很少。这些结果显示两种佐剂诱导的EAE没有本质区别。（5）在用Tbc佐剂免疫的少数动物中，除CF抗体外，后来还出现了可检测到的PH抗体。这些动物接受了BP皮肤试验，并且体重减轻或肢体无力已恢复。结果表明，体液抗体在改变疾病进程中起作用，即使它们对EAE的产生不是必需的。
