Sharma T, Das B C
Mutat Res. 1981 May;81(3):357-64. doi: 10.1016/0027-5107(81)90122-6.
Various concentrations of 5-bromodeoxyuridine (BdrU) ranging from 0.01 to 10.0 microgram/ml were tried for finding the minimal concentration required for differential staining of sister chromatids in lymphocytes of man, muntjac and cattle grown in three commonly used culture media, namely TC 199, Dulbecco's MEM and Ham's F-10. The lymphocytes grown in TC 199 required the lowest concentration of BrdU whereas it was highest for the lymphocytes grown in F-10. The minimal concentration varied for the 3 species studied, and it was not related to their DNA content. The differing amounts of thymidine and folic acid present in the various culture media seemed to account for the difference in the quantity of BdrU required for eliciting differential staining. Staining may also have depended on the intracellular nucleotide pool and/or on the difference in the substitutable dT sites of the genomes.
尝试了浓度范围从0.01至10.0微克/毫升的各种5-溴脱氧尿苷（BrdU），以找出在三种常用培养基（即TC 199、杜尔贝科改良伊格尔培养基和哈姆F-10培养基）中培养的人、麂和牛的淋巴细胞中姐妹染色单体进行差异染色所需的最低浓度。在TC 199中培养的淋巴细胞所需的BrdU浓度最低，而在F-10中培养的淋巴细胞所需浓度最高。所研究的3个物种的最低浓度各不相同，且与它们的DNA含量无关。各种培养基中胸腺嘧啶核苷和叶酸含量的差异似乎可以解释引发差异染色所需BrdU量的不同。染色也可能取决于细胞内核苷酸库和/或基因组中可替代dT位点的差异。
