O'Donnell I J, Green P E, Connell J A, Hopkins P S
Aust J Biol Sci. 1981;34(4):411-7. doi: 10.1071/bi9810411.
Sheep were immunized with antigens extracted from third-instar larvae of L. cuprina. This procedure produced substantial titres of circulating antibody as measured by solid-phase radioimmunoassay or immunodiffusion or by both techniques. However, immunization did not confer protection against subsequent implant challenge with first-instar larvae. In vitro studies indicated that pooled sera from immunized sheep (mean immunodiffusion titre = 3) significantly reduced larval survival. Antigen specificity and the modulating effects of concomitant humoral responses to larval challenge are discussed in relation to the findings.
用从铜绿丽蝇三龄幼虫中提取的抗原对绵羊进行免疫。通过固相放射免疫测定法、免疫扩散法或两种技术测定，该程序产生了大量循环抗体。然而，免疫并未赋予对随后一龄幼虫植入攻击的保护作用。体外研究表明，来自免疫绵羊的混合血清（平均免疫扩散滴度 = 3）显著降低了幼虫存活率。结合研究结果讨论了抗原特异性以及对幼虫攻击的伴随体液反应的调节作用。
