East I J, Fitzgerald C J, Pearson R D, Donaldson R A, Vuocolo T, Cadogan L C, Tellam R L, Eisemann C H
CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Queensland, Australia.
Int J Parasitol. 1993 Apr;23(2):221-9. doi: 10.1016/0020-7519(93)90144-n.
A culture system has been established to produce gram amounts of peritrophic membrane from larvae of the sheep blowfly, Lucilia cuprina. Peritrophic membrane obtained from this culture has been used to immunize sheep. The immunization produced an immune response which resulted in the average weight of larvae on immunized sheep being only 50% of that of larvae grown on control sheep (P < 0.05). Fractionation of the components of the peritrophic membrane followed by immunization trials showed that the protective antigen fraction comprised material that could only be solubilized by harsh agents such as 4 M-urea. Even after solubilization by 4 M-urea, the protective antigens were able to produce a protective immune response which reduced growth of larvae on immunized sheep to 55% of larvae grown on control sheep (P < 0.05). This immune response which reduced growth of the larvae did not cause gross morphological damage to the larvae.
