White F P
Brain Res. 1981 Dec 14;229(1):43-52. doi: 10.1016/0006-8993(81)90744-7.
The distribution of protein and ribonucleic acid (RNA) synthesized by telencephalon slices was determined by radioautography at the light microscope level. Previous studies had shown that, in telencephalon slices, the cells associated with the cerebral microvasculature were synthesizing protein at very high rates compared to other cerebral cells such as neurons. In this study, therefore, particular emphasis was placed on the distribution of newly synthesized protein and RNA among the various cell types of the microvasculature. Rat telencephalon slices were incubated with either [3H] leucine or [3H] uridine for 1 h. The 3H-protein that was found in association with microvessels was present in endothelial cells, pericytes and smooth muscles cells. There was also extensive labeling of cellular processes adjacent to the abluminal side of the basement membrane and extending as far as 15 micrometer from the lumen. The latter processes may have been astrocyte glial projections. All [3H] RNA found associated with microvessels was, however, restricted to endothelial cells and pericytes. This data is discussed in relation to the induction of a 71,000 dalton protein which occurs within minutes of the preparation of tissue slices, and requires the synthesis of new RNA.
通过光学显微镜水平的放射自显影术确定端脑切片合成的蛋白质和核糖核酸（RNA）的分布。先前的研究表明，在端脑切片中，与脑微血管相关的细胞与其他脑细胞（如神经元）相比，合成蛋白质的速率非常高。因此，在本研究中，特别强调了新合成的蛋白质和RNA在微血管各种细胞类型中的分布。将大鼠端脑切片与[3H]亮氨酸或[3H]尿苷孵育1小时。在微血管中发现的3H-蛋白质存在于内皮细胞、周细胞和平滑肌细胞中。在基底膜腔外侧相邻的细胞突起中也有广泛的标记，并且从管腔延伸至15微米远。后者的突起可能是星形胶质细胞的神经胶质突起。然而，所有与微血管相关的[3H]RNA都仅限于内皮细胞和周细胞。结合在组织切片制备后几分钟内出现的一种71,000道尔顿蛋白质的诱导情况对该数据进行了讨论，并且这种诱导需要新RNA的合成。
