Pavlík A, Jakoubek B
Brain Res. 1976 Jan 9;101(1):113-28. doi: 10.1016/0006-8993(76)90992-6.
The distribution of protein-bound labelled leucine in brain cortex slices, prepared from adult rats by various methods and incubated with [14C]- or [3H]leucine, was investigated by autoradiography. In the first and second slices a marked gradient of incorporated radioactivity from the cut surface to the slice interior was observed. Very high labelling of leptomeningeal cells and vessels enhanced further the inhomogeneity of radioactivity distribution. Light microscopic examination of incubated slices revealed morphological alterations of neurones, especially in the vicinity of the cut surface. The comparison of grain density over neurones and their satellite glia indicated markedly higher incorporation into the latter. The ATP level in slices at the end of incubation reflected the method of slice preparation and morphological integrity. Inhomogeneity of incorporated radioactivity distribution in brain slices contrasted with the uniform labelling of cortical cells in vivo, and may represent at least one reason for the low estimates of protein synthesis rate in brain cortex slices.
通过放射自显影术研究了用各种方法从成年大鼠制备的脑皮质切片中蛋白质结合的标记亮氨酸的分布，这些切片与[14C]-或[3H]亮氨酸一起孵育。在第一和第二切片中，观察到从切割表面到切片内部掺入放射性的明显梯度。软脑膜细胞和血管的非常高的标记进一步增强了放射性分布的不均匀性。对孵育切片的光学显微镜检查揭示了神经元的形态改变，特别是在切割表面附近。对神经元及其卫星胶质细胞上颗粒密度的比较表明，后者的掺入明显更高。孵育结束时切片中的ATP水平反映了切片制备方法和形态完整性。脑切片中掺入放射性分布的不均匀性与体内皮质细胞的均匀标记形成对比，这可能是脑皮质切片中蛋白质合成速率估计值较低的至少一个原因。
