Hershenfeld S, Kazdan J J, Mancer K, Feugas P, Basu P K, Avaria M
Can J Ophthalmol. 1981 Apr;16(2):76-8.
A technique is described for studying the cellular response in various conjunctival disorders. A round plastic disc (Thermanox) is pressed against the patient's conjunctive, and the material adhering to the disc is stained with a modified Wright's stain (Diff-Quik) for light microscopy. This method compares favourably with the classic conjunctival scraping method. The advantages of the impression technique are that no local anesthetic is needed for abtaining conjunctival samples, it is painless and can readily be used in children, repeated samples can be taken within a short time, the staining procedure can be completed within 15 seconds, and the discs can be processed for electron microscopic studies of the cellular samples.
本文描述了一种用于研究各种结膜疾病细胞反应的技术。将一个圆形塑料盘(Thermanox)压在患者的结膜上,粘附在盘上的物质用改良的瑞氏染色剂(Diff-Quik)染色,用于光学显微镜检查。该方法与经典的结膜刮片法相比具有优势。印片技术的优点是获取结膜样本时无需局部麻醉,无痛且可轻松用于儿童,可在短时间内重复取样,染色过程可在15秒内完成,并且这些圆盘可用于对细胞样本进行电子显微镜研究。