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结膜炎的印片细胞学检查

Impression cytology in conjunctivitis.

作者信息

Hershenfeld S, Kazdan J J, Mancer K, Feugas P, Basu P K, Avaria M

出版信息

Can J Ophthalmol. 1981 Apr;16(2):76-8.

PMID:6171332
Abstract

A technique is described for studying the cellular response in various conjunctival disorders. A round plastic disc (Thermanox) is pressed against the patient's conjunctive, and the material adhering to the disc is stained with a modified Wright's stain (Diff-Quik) for light microscopy. This method compares favourably with the classic conjunctival scraping method. The advantages of the impression technique are that no local anesthetic is needed for abtaining conjunctival samples, it is painless and can readily be used in children, repeated samples can be taken within a short time, the staining procedure can be completed within 15 seconds, and the discs can be processed for electron microscopic studies of the cellular samples.

摘要

本文描述了一种用于研究各种结膜疾病细胞反应的技术。将一个圆形塑料盘(Thermanox)压在患者的结膜上,粘附在盘上的物质用改良的瑞氏染色剂(Diff-Quik)染色,用于光学显微镜检查。该方法与经典的结膜刮片法相比具有优势。印片技术的优点是获取结膜样本时无需局部麻醉,无痛且可轻松用于儿童,可在短时间内重复取样,染色过程可在15秒内完成,并且这些圆盘可用于对细胞样本进行电子显微镜研究。

相似文献

1
Impression cytology in conjunctivitis.结膜炎的印片细胞学检查
Can J Ophthalmol. 1981 Apr;16(2):76-8.
2
Conjunctival impression cytology.结膜印迹细胞学检查。
Arch Ophthalmol. 1977 Apr;95(4):678-81. doi: 10.1001/archopht.1977.04450040144022.
3
Conjunctival impression cytology in dogs.犬结膜印迹细胞学检查
Vet Ophthalmol. 2005 Nov-Dec;8(6):401-5. doi: 10.1111/j.1463-5224.2005.00414.x.
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Impression cytology of the conjunctival epithelium in patients with vernal conjunctivitis.春季结膜炎患者结膜上皮的印片细胞学检查
Eye (Lond). 1996;10 ( Pt 1):82-5. doi: 10.1038/eye.1996.12.
5
[Clinical and cytological parallels in inflammatory diseases of the conjunctiva].[结膜炎症性疾病的临床与细胞学对照]
Vestn Oftalmol. 1997 Jan-Feb;113(1):33-6.
6
Diagnostic cytology in some conjunctival diseases.
Bull Ophthalmol Soc Egypt. 1976;69(73):27-37.
7
Diagnostic impression cytology: a simple technique for the diagnosis of external eye disease.
Cytopathology. 1997 Dec;8(6):373-80. doi: 10.1111/j.1365-2303.1997.tb00567.x.
8
Direct immunofluorescence and scraping conjunctival cytology in the study of 912 patients affected by microfollicular conjunctivitis.
Ophthalmologica. 1994;208(6):295-7. doi: 10.1159/000310523.
9
Evaluation of goblet cells in conjunctival impression cytology by multiple-step & two-step staining techniques.采用多步染色技术和两步染色技术评估结膜印迹细胞学中的杯状细胞。
Indian J Med Res. 1989 Jun;90:233-6.
10
Staging of conjunctival squamous metaplasia by impression cytology.
Ophthalmology. 1985 Jun;92(6):728-33. doi: 10.1016/s0161-6420(85)33967-2.

引用本文的文献

1
Fluorescein punctate staining traced to superficial corneal epithelial cells by impression cytology and confocal microscopy.印迹细胞学和共聚焦显微镜检查发现荧光素点状染色位于浅层角膜上皮细胞。
Invest Ophthalmol Vis Sci. 2011 Apr 5;52(5):2127-35. doi: 10.1167/iovs.10-6489. Print 2011 Apr.
2
Polycarbonate membrane impression cytology: evidence for fluorescein staining in normal and dry eye corneas.聚碳酸酯膜印片细胞学:正常和干眼眼角膜中荧光素染色的证据。
Br J Ophthalmol. 2010 Apr;94(4):406-9. doi: 10.1136/bjo.2009.167031.
3
Effect of hydrocortisone on the mobilisation of leucocytes in corneal wounds.
氢化可的松对角膜伤口中白细胞动员的影响。
Br J Ophthalmol. 1981 Oct;65(10):694-8. doi: 10.1136/bjo.65.10.694.