Aragona P, Romeo G F, Puzzolo D, Micali A, Ferreri G
Institute of Ophthalmology, Biomorphology, University of Messina, Italy.
Eye (Lond). 1996;10 ( Pt 1):82-5. doi: 10.1038/eye.1996.12.
The alterations in the conjunctival epithelium during the course of vernal conjunctivitis were examined by conjunctival impression cytology. The study was carried out on 53 patients with vernal conjunctivitis and 20 normal subjects as control. The results of impression cytology demonstrated that all cytological parameters were significantly modified in vernal conjunctivitis patients; the earliest alterations were found in the distribution of goblet cells, in the intercellular junctions, in the chromatin morphology and in the degree of keratinisation. The morphometric comparison showed that in vernal conjunctivitis patients the mean number of goblet cells per field was significantly higher than in controls. Moreover the mean diameter of goblet cells was smaller in patients than in controls. Impression cytology can, therefore, be a simple, non-invasive and cheap method for the study of the ocular surface in vernal conjunctivitis.
通过结膜印片细胞学检查了春季结膜炎病程中结膜上皮的改变。研究对53例春季结膜炎患者和20名正常受试者作为对照进行。印片细胞学结果表明，春季结膜炎患者的所有细胞学参数均有显著改变；最早发现的改变见于杯状细胞分布、细胞间连接、染色质形态和角化程度。形态计量学比较显示，春季结膜炎患者每视野杯状细胞的平均数量显著高于对照组。此外，患者杯状细胞的平均直径小于对照组。因此，印片细胞学可作为一种简单、无创且廉价的方法用于研究春季结膜炎的眼表。
