Deutsch J, Gilon C, Chorev M
Int J Pept Protein Res. 1981 Aug;18(2):203-7.
In this article we present preliminary results of the application of potassium cationized field desorption mass spectrometry as an additional technique for the elucidation of structure and evaluation of purity of oligopeptides such as C-terminal penta- and hexapeptide analogs of substance P. In the resultant mass spectra both a protonated and a cationized molecular ion, MH+ and MK+ respectively, were observed. The m/z values of the two peaks were in agreement with the calculated molecular weights. The ratio between the relative abundancies of these ions (MH+/MK+) was found to be characteristic of the particular peptide and thus useful in the assessment of their purity. Among the 13 peptides studied, only two gave pyrolytic fragmentation leading to a more complex spectra.
在本文中，我们展示了钾离子化场解吸质谱法作为一种辅助技术用于阐明诸如P物质C端五肽和六肽类似物等寡肽结构及评估其纯度的初步结果。在所得质谱图中，分别观察到了质子化分子离子和阳离子化分子离子，即MH⁺和MK⁺。这两个峰的m/z值与计算得到的分子量一致。发现这些离子的相对丰度之比（MH⁺/MK⁺）是特定肽的特征，因此有助于评估其纯度。在所研究的13种肽中，只有两种产生了热解碎片，导致质谱图更为复杂。
