Buko A M, Sarin V K
Department of Analytical Research, Abbott Laboratories, Abbott Park, IL 60064.
Rapid Commun Mass Spectrom. 1990 Dec;4(12):541-5. doi: 10.1002/rcm.1290041215.
252Cf plasma-desorption mass spectrometry (PDMS) has been demonstrated to provide sequence-specific fragmentation for several oligopeptides. The nature of the fragment ions observed is generally similar to that observed using liquid secondary-ion mass spectrometry (LSIMS) and can be observed using less sample than LSIMS requires, but PDMS spectra are acquired at a lower resolution. In addition, the molecular weight of some of the oligopeptides studied exceeds that which is generally accepted as within the sequence range of LSIMS. The specific series of sequence ions that predominate in the PDMS spectra appear to be related to the amino acid compositions and sequences of the oligopeptides.
已证明252Cf等离子体解吸质谱法（PDMS）可为多种寡肽提供序列特异性裂解。观察到的碎片离子的性质通常与使用液体二次离子质谱法（LSIMS）观察到的相似，并且所需样品量比LSIMS少，但PDMS光谱是在较低分辨率下采集的。此外，所研究的一些寡肽的分子量超过了通常认为在LSIMS序列范围内的分子量。PDMS光谱中占主导地位的特定序列离子系列似乎与寡肽的氨基酸组成和序列有关。
