Guillouzo A, Weber A, Le Provost E, Rissel M, Schapira F
J Cell Sci. 1981 Jun;49:249-60. doi: 10.1242/jcs.49.1.249.
Cellular and subcellular immunolocalization of aldose isozymes and alpha-foetoprotein (AFP) was performed in rat liver during the different stages of carcinogenesis induced by 3'-methyl-4-dimethylaminoazobenzene. During the early stages, double-labelling experiments showed that oval and transitional cells that expressed foetal aldolases did not contain adult aldolase B; this isozyme was only found in small and "normal' hepatocytes. AFP was present in transitional cells and in small hepatocytes. During hyperplastic nodule development, neither foetal aldolases nor AFP were located in hepatocytes. These foetal proteins were still observed in transitional cells. In hepatocellular carcinomas, both foetal proteins (aldolase isozymes and AFP) and adult aldolase B were present in malignant cells. Moreover, during the different stages foetal aldolases were also found in sinusoidal cells. These results indicate that, during azo-dye hepatocarcinogenesis, (a) several cell types synthesize foetal aldolases: oval and transitional cells, hepatoma cells and sinusoidal cells; (b) only hepatoma cells and not hepatocytes located in hyperplastic nodules can express both foetal and adult aldolases. This suggests that in primary, as in transplanted, hepatoma the resurgence of foetal isozymes is the consequence of a disturbance of control gene expression.
在由3'-甲基-4-二甲基氨基偶氮苯诱导的大鼠肝脏致癌作用的不同阶段，对醛糖同工酶和甲胎蛋白（AFP）进行了细胞和亚细胞免疫定位。在早期阶段，双重标记实验表明，表达胎儿醛缩酶的卵圆细胞和过渡细胞不含成人醛缩酶B；这种同工酶仅在小的“正常”肝细胞中发现。AFP存在于过渡细胞和小肝细胞中。在增生性结节发展过程中，胎儿醛缩酶和AFP均不在肝细胞中。在过渡细胞中仍可观察到这些胎儿蛋白。在肝细胞癌中，恶性细胞中同时存在胎儿蛋白（醛缩酶同工酶和AFP）和成人醛缩酶B。此外，在不同阶段，在窦状细胞中也发现了胎儿醛缩酶。这些结果表明，在偶氮染料诱导的肝癌发生过程中，（a）几种细胞类型合成胎儿醛缩酶：卵圆细胞和过渡细胞、肝癌细胞和窦状细胞；（b）只有肝癌细胞而非增生性结节中的肝细胞能够同时表达胎儿和成人醛缩酶。这表明，在原发性肝癌以及移植性肝癌中，胎儿同工酶的重现是控制基因表达紊乱的结果。
