Groome N P
J Clin Chem Clin Biochem. 1981 Oct;19(10):1067-73. doi: 10.1515/cclm.1981.19.10.1067.
The assay of myelin basic protein in body fluids has potential clinical importance as a routine indicator of demyelination. Preliminary details of a competitive enzyme immunoassay for this protein have previously been published by the author (Groome, N. P. (1980) J. Neurochem. 35, 1409-1417). The present paper now describes the adaptation of this assay for use on human plasma and various aspects of routine data processing. A commercially available cuvette system was found to have advantages over microtitre plates but required a permuted arrangement of sample replicates for consistent results. For dose interpolation, the standard curve could be fitted to a three parameter non-linear equation by regression analysis or linearised by the logit/log transformation.
体液中髓鞘碱性蛋白的检测作为脱髓鞘的常规指标具有潜在的临床重要性。作者之前已发表了该蛋白竞争性酶免疫测定的初步细节（格鲁姆，N.P.（1980年）《神经化学杂志》35卷，第1409 - 1417页）。本文现描述了该测定法在人血浆中的应用调整以及常规数据处理的各个方面。发现一种市售的比色皿系统比微量滴定板具有优势，但需要对样品重复进行置换排列以获得一致的结果。对于剂量插值，标准曲线可通过回归分析拟合到一个三参数非线性方程，或通过对数几率/对数变换进行线性化。
