Ohta Mitsuhiro, Ohta Kiyoe, Nishimura Masataka, Saida Takahiko
Clinical Research Center, Utano National Hospital, Kyoto 616-8255, Japan.
Ann Clin Biochem. 2002 Nov;39(Pt 6):603-5. doi: 10.1177/000456320203900610.
Human T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy (HAM)/tropical spastic paraparesis (TSP) is a chronic inflammatory disease that primarily affects the spinal cord and may be a neurological syndrome that is clinically similar to multiple sclerosis (MS). Myelin basic protein (MBP) in the cerebrospinal fluid (CSF) of MS patents is generally measured by radioimmunoassay. We have recently established a sensitive enzyme-linked immunosorbent assay (ELISA) and measured the MBP concentrations in CSF and serum of HAM/TSP patients.
A sensitive two-site ELISA capable of measuring MBP at a concentration as low as 30 pg/mL in serum and CSF samples was used.
Significantly higher CSF MBP concentrations were detected in 61% of HAM/TSP patients than in patients with non-neurological diseases. Serum MBP concentrations were also higher in 9% of HAM/TSP patients compared with patients with non-neurological diseases or healthy controls.
Using our ELISA system, we detected MBP in CSF and serum not only in patients with central active demyelination as in MS, but also in patients with spinal cord demyelination as in HAM/TSP.
人类嗜T淋巴细胞病毒1型（HTLV-1）相关脊髓病（HAM）/热带痉挛性截瘫（TSP）是一种主要影响脊髓的慢性炎症性疾病，可能是一种临床上与多发性硬化症（MS）相似的神经综合征。MS患者脑脊液（CSF）中的髓鞘碱性蛋白（MBP）通常通过放射免疫测定法进行检测。我们最近建立了一种灵敏的酶联免疫吸附测定法（ELISA），并测量了HAM/TSP患者脑脊液和血清中的MBP浓度。
使用一种灵敏的双位点ELISA，该方法能够在血清和脑脊液样本中检测低至30 pg/mL浓度的MBP。
在61%的HAM/TSP患者中检测到脑脊液MBP浓度显著高于非神经疾病患者。与非神经疾病患者或健康对照相比，9%的HAM/TSP患者血清MBP浓度也更高。
使用我们的ELISA系统，我们不仅在MS等中枢性活动性脱髓鞘患者的脑脊液和血清中检测到了MBP，而且在HAM/TSP等脊髓脱髓鞘患者中也检测到了MBP。
