Donald A S
Eur J Biochem. 1981 Nov;120(2):243-9. doi: 10.1111/j.1432-1033.1981.tb05695.x.
Blood-group-specific glycoproteins obtained from ovarian cyst fluids of A1 and A2 persons were degraded with NaOH/NaBH4. The oligosaccharides released were de-N-acetylated with Ba(OH)2 and then hydrolysed with dilute H2SO4. The products were fractionated on columns of ion-exchange resin and the components isolated were re-N-acetylated with 14C-labelled acetic anhydride; further purification was effected by paper chromatography. The following trisaccharides: type 1, GalNAc(alpha 1-3)Gal(beta 1-3)GlcNAc; type 2, GalNAc(alpha 1-3)-Gal(beta 1-4)GlcNAc; type 3 (reduced), GalNAc(alpha 1-3)Gal (beta 1-3)GalNAcOH (where Gal is galactose; GalNAc is N-acetylgalactosamine, GlcNAc is N-acetylglucosamine and GalNAcOH is N-acetylgalactosaminitol) were isolated and characterised from both the A1 and A2 materials. The type 3 (reduced) trisaccharide has not previously been obtained from human glycoproteins. Chromatographic evidence indicated that the three trisaccharide structures were also present in other A1, A2, A1B and A2B ovarian cyst glycoproteins and in A1 and A2 salivary glycoproteins. These findings are not indicative of structural differences between the A determinants of A1 and A2 glycoproteins.
从A1型和A2型个体的卵巢囊肿液中获得的血型特异性糖蛋白用NaOH/NaBH4降解。释放出的寡糖用Ba(OH)2进行脱N-乙酰化，然后用稀H2SO4水解。产物在离子交换树脂柱上进行分级分离，分离出的组分用14C标记的乙酸酐进行再N-乙酰化；通过纸色谱法进一步纯化。从A1型和A2型物质中分离并鉴定出以下三种三糖：1型，GalNAc(α1-3)Gal(β1-3)GlcNAc；2型，GalNAc(α1-3)-Gal(β1-4)GlcNAc；3型（还原型），GalNAc(α1-3)Gal(β1-3)GalNAcOH（其中Gal是半乳糖；GalNAc是N-乙酰半乳糖胺，GlcNAc是N-乙酰葡糖胺，GalNAcOH是N-乙酰半乳糖胺醇）。3型（还原型）三糖以前从未从人糖蛋白中获得过。色谱证据表明，这三种三糖结构也存在于其他A1、A2、A1B和A2B卵巢囊肿糖蛋白以及A1和A2唾液糖蛋白中。这些发现并不表明A1和A2糖蛋白的A决定簇之间存在结构差异。
