Kovárů H, Kovárů F
Folia Microbiol (Praha). 1981;26(5):417-21. doi: 10.1007/BF02927338.
An immunomodulatory effect of pig alpha-fetoprotein (AFP) on early phytohaemagglutinin (PHA)-stimulated intact thymocytes from piglets was analyzed. The activity of surface-localized Na+, K+-adenosinetriphosphatase was estimated, as the early reaction of mitogen-stimulated T-lymphocytes. A high inhibition of PHA-stimulated enzyme activity was found in the presence of AFP. The reaction was dependent of PHA concentration when using a constant AFP concentration. The highest AFP-induced decrease in PHA-induced Na+, K+-ATPase activity was 72%. AFP was isolated by a new technique based on salting out in ammonium sulphate and acetate buffer solutions. A gentle, simple and rapid procedure makes it possible to obtain AFP in a high degree of purity with preserved biological activity. The presented results can contribute to understanding the molecular aspects of AFP immunoregulation of lymphocyte reactivity in vitro.
分析了猪甲胎蛋白（AFP）对早期受植物血凝素（PHA）刺激的仔猪完整胸腺细胞的免疫调节作用。作为有丝分裂原刺激的T淋巴细胞的早期反应，对表面定位的Na +，K + - 三磷酸腺苷酶的活性进行了评估。在存在AFP的情况下，发现PHA刺激的酶活性受到高度抑制。当使用恒定的AFP浓度时，该反应取决于PHA浓度。AFP诱导的PHA诱导的Na +，K + -ATP酶活性的最大降低为72％。通过基于在硫酸铵和乙酸盐缓冲溶液中盐析的新技术分离AFP。温和、简单且快速的程序使得能够以高纯度获得具有保留生物活性的AFP。所呈现的结果有助于理解体外AFP对淋巴细胞反应性免疫调节的分子方面。
