Petrás S, Krutsay M
Morphol Igazsagugyi Orv Sz. 1981 Oct;21(4):303-6.
Authors studied the effect of various decalcifying solutions on the staining of cell nuclei. It remained normal within the period necessary for decalcification only when n-hydrochloric acid containing 2 per cent of ferric chloride or solution of EDTA III were used. Decalcification of solid bone specimens in hydrochloride acid-ferric chloride solution at room temperature can be completed in 6-7 days. The staining of cell nuclei remains normal by the 12th day. A satisfactory effect--decalcification in 14 days without any damage to the cell nuclei staining--can be achieved by a 27 per cent, pH 7.4 solution of EDTA III at a temperature 60 degrees C.
作者研究了各种脱钙溶液对细胞核染色的影响。仅当使用含2%氯化铁的正盐酸或EDTA III溶液时，在脱钙所需的时间内细胞核仍保持正常。室温下，固体骨标本在盐酸-氯化铁溶液中脱钙可在6-7天内完成。到第12天时，细胞核染色仍保持正常。在60摄氏度下，27%、pH值7.4的EDTA III溶液可在14天内实现脱钙且对细胞核染色无任何损害，效果令人满意。
