[制作牙齿及其附属牙周组织联合切片的脱钙方法研究]
[Study of methods of decalcification for making united slices of tooth and affiliated periodontic tissues].
作者信息
Wang Yu, Mu Ya-bing, Miao Lei-ying, Sun Hong-chen, Li Cheng-ku
机构信息
Department of Pathology, School of Stomatology, Jilin University, Changchun 130041, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Mar;42(3):150-1.
OBJECTIVE
To study the methods of decalcification for making united slices of tooth and affiliated periodontic tissues.
METHODS
Twenty-one samples containing dog molars and affiliated periodontic tissues were divided into seven mean groups. The pH value of solution, time of decalcification, weight and volume of samples, and content of decalcified calcium were detected. The slices were observed by HE, specific, and immunohistochemical stain.
RESULTS
The velocity of decalcification increased with decrease of solution pH. The weight of samples lightened by 37.61%, the volume reduced by 25.97% on average, and calcium decalcified was 174.49 mg per gram humid samples. The EDTA decalcification was slowest, but it was best. Decalcification was fast in Plank-Rycho solution while the section was worst, and faster in the formyl solution containing aluminium chloride than in EDTA, and the section was better.
CONCLUSIONS
The 50% formyl solution containing aluminium chloride is an ideal decalcifying solution.
目的
研究制作牙齿及其附属牙周组织联合切片的脱钙方法。
方法
将21个包含犬磨牙及其附属牙周组织的样本平均分为7组。检测溶液的pH值、脱钙时间、样本重量和体积以及脱钙钙含量。切片经苏木精-伊红（HE）染色、特殊染色和免疫组织化学染色观察。
结果
脱钙速度随溶液pH值降低而加快。样本重量平均减轻37.61%，体积平均减少25.97%，每克湿样本脱钙钙量为174.49毫克。乙二胺四乙酸（EDTA）脱钙最慢，但效果最佳。普兰克-里乔（Plank-Rycho）溶液脱钙快，但切片效果最差，含氯化铝的甲酰溶液脱钙比EDTA快，且切片效果较好。
结论
含氯化铝的50%甲酰溶液是理想的脱钙溶液。
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