Nagase M, Honda N
Ren Physiol. 1980;3(1-6):244-9. doi: 10.1159/000172767.
The effect of reduced glomerular anionic charge on immune complex deposition in the glomerular basement membrane was studied in rats with Heymann nephritis. A reduction of the anionic charge was induced by lysozyme administration. The animals were divided into the following four groups: normal controls (group A), group B treated with lysozyme, group C treated with lysozyme and immunized with autologous tubular antigen, and group D immunized with the antigen. Proteinuria was marked in groups B, C and D as compared with group A, especially in group D. Development of Heymann nephritis by the tubular antigen was suppressed in group C. Stainings of anionic substances in the glomerular capillary walls by cationic probes were weakened in groups B, C and D. Immune complex deposited in the subepithelial area of the capillary wall was also stained by cationic probes. The findings suggest that reduced glomerular anionic charge by polycations results in suppressed immune complex deposition in the basement membrane, partially due to increased permeation of macromolecules across the glomerular capillary walls in rats.
在患有海曼肾炎的大鼠中，研究了肾小球阴离子电荷减少对免疫复合物在肾小球基底膜沉积的影响。通过给予溶菌酶诱导阴离子电荷减少。将动物分为以下四组：正常对照组（A组）、接受溶菌酶治疗的B组、接受溶菌酶治疗并注射自体肾小管抗原的C组、以及注射抗原的D组。与A组相比，B、C、D组出现明显蛋白尿，尤其是D组。C组中肾小管抗原诱发的海曼肾炎发展受到抑制。B、C、D组中阳离子探针显示肾小球毛细血管壁中阴离子物质的染色减弱。阳离子探针也可对沉积在毛细血管壁上皮下区域的免疫复合物进行染色。这些发现表明，多阳离子导致的肾小球阴离子电荷减少会抑制免疫复合物在基底膜中的沉积，部分原因是大鼠肾小球毛细血管壁上大分子的通透性增加。
