Ohtsuka A, Murakami T, Irino S, Jones A L
Scan Electron Microsc. 1981(Pt 2):83-6, 82.
Gelatin-coated glass cover slips were immersed in a mixture of glutaraldehyde and tannic acid, and rinsed in bovine or human serum. The gelatin-coated cover slips were first placed in a human blood cell suspension, and subsequently placed in a solution of glutaraldehyde or osmium tetroxide. This preliminary mounting of the blood cells eliminated troublesome centrifugations in the tannin-osmium-thiocarbohydrazide-osmium (TaOTO) impregnation. The blood cells seldom detached from the cover slips even when the specimens were dehydrated with ethanol and critical point dried with liquid carbon dioxide. Both the mounting media as well as the mounted cells were intensely stained by the TaOTO method, and exhibited no charging in the scanning electron microscope even at acceleration voltages of 25 kV. The scanning images of the white blood cells with their microvilli were clearly visible with sharp contrast.
明胶包被的盖玻片浸入戊二醛和鞣酸的混合液中，然后在牛血清或人血清中冲洗。将明胶包被的盖玻片先置于人血细胞悬液中，随后置于戊二醛或四氧化锇溶液中。血细胞的这种初步固定消除了单宁 - 锇 - 硫代碳酰肼 - 锇（TaOTO）浸渍过程中麻烦的离心步骤。即使在用乙醇脱水并用液态二氧化碳进行临界点干燥时，血细胞也很少从盖玻片上脱落。固定介质和固定的细胞都被TaOTO方法强烈染色，并且即使在25 kV的加速电压下在扫描电子显微镜中也没有显示出电荷。带有微绒毛的白细胞的扫描图像清晰可见，对比度鲜明。
