体外刺激抗肝素RNA聚合酶I转录复合体。
Stimulation in vitro of a heparin-resistant RNA polymerase I transcription complex.
作者信息
Haddox M K, Russell D H
出版信息
Biochem J. 1981 Jul 15;198(1):207-10. doi: 10.1042/bj1980207.
Abstract
A soluble factor partially purified from calf liver increases transcription by RNA polymerase I in isolated nuclei. Addition of the factor to reactions which have reached a plateau owing to the inability to reinitiate on the endogenous chromatin template restores the initial rate of synthesis and stimulates an increased accumulation of RNA product. The RNA synthesis stimulated by factor addition is identical with that initiated in vivo in that it is resistant to heparin disruption.
摘要
从小牛肝脏中部分纯化得到的一种可溶性因子可增加分离细胞核中RNA聚合酶I的转录。将该因子添加到由于无法在内源性染色质模板上重新起始而达到平台期的反应中，可恢复初始合成速率并刺激RNA产物积累增加。添加因子所刺激的RNA合成与体内起始的合成相同，因为它对肝素破坏具有抗性。
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