Tabacik C, Aliau S, Astruc M, De Paulet A C
Biochim Biophys Acta. 1981 Dec 23;666(3):433-41. doi: 10.1016/0005-2760(81)90303-9.
Cholesterol biosynthesis was evaluated in different parts of the human gastro-intestinal tract by incorporation of labelled acetic or mevalonic acid into non-saponifiable metabolites and also by measuring squalene epoxidase activity, oxido=squalene cyclase activity and dihydrolanosterol demethylation rate. With respect to rat liver, whole cholesterol biosynthesis was generally low and we found an anatomic localization of activity towards the end of the gastro-intestinal tract. The results of analysis of mevalonic acid metabolites implied the existence of post-HMG CoA regulation. A comparison with the corresponding dat obtained from homologous cancerous tissues (inthe case of high cholesterogenic activity) did not show a greater activity in the tumor, in spite of higher levels of epoxidase concentration.
通过将标记的乙酸或甲羟戊酸掺入非皂化代谢物中，并通过测量角鲨烯环氧化酶活性、氧化角鲨烯环化酶活性和二氢羊毛甾醇去甲基化率，评估了人胃肠道不同部位的胆固醇生物合成。与大鼠肝脏相比，整个胆固醇生物合成通常较低，并且我们发现活性在胃肠道末端存在解剖学定位。甲羟戊酸代谢物的分析结果表明存在HMG CoA后调节。与从同源癌组织（在高胆固醇生成活性的情况下）获得的相应数据进行比较，尽管环氧化酶浓度较高，但肿瘤中并未显示出更高的活性。
