新生雏鸡组织中由乙酸盐合成角鲨烯、羊毛甾醇和胆固醇

Squalene, lanosterol and cholesterol synthesis from acetate in neonatal chick tissues.

作者信息

Linares A, Arce V, Aguilera J A, García-Peregrín E

出版信息

Rev Esp Fisiol. 1984 Dec;40(4):425-9.

Abstract

Acetate incorporation into squalene, lanosterol and cholesterol by liver and kidney slices and intestinal mucosa scrapes from neonatal chick was studied. Contrary to what is observed when using mevalonate as substrate, cholesterol was the main nonsaponifiable synthesized from acetate in all the conditions assayed. Low percentages of squalene and lanosterol were synthesized by liver and kidney slices, while in intestinal mucosa squalene was practically undetectable. The highest percentage of radioactivity in cholesterol was found in liver, followed by intestinal mucosa and kidney. Relative percentages of squalene, lanosterol and cholesterol were practically similar in each tissue at any incubation time and acetate concentration considered. Only in kidney these percentages, especially in the case of squalene, seemed to decrease at higher acetate concentrations (8-12 mM).

摘要

研究了新生雏鸡的肝脏和肾脏切片以及肠黏膜刮片将乙酸盐掺入角鲨烯、羊毛甾醇和胆固醇的情况。与使用甲羟戊酸作为底物时观察到的情况相反，在所有测定条件下，胆固醇都是由乙酸盐合成的主要非皂化物质。肝脏和肾脏切片合成的角鲨烯和羊毛甾醇比例较低，而在肠黏膜中几乎检测不到角鲨烯。肝脏中胆固醇的放射性百分比最高，其次是肠黏膜和肾脏。在任何孵育时间和所考虑的乙酸盐浓度下，每个组织中角鲨烯、羊毛甾醇和胆固醇的相对百分比实际上相似。只有在肾脏中，这些百分比，尤其是角鲨烯的百分比，在较高乙酸盐浓度（8 - 12 mM）下似乎会降低。