Thomas T L, Posakony J W, Anderson D M, Britten R J, Davidson E H
Chromosoma. 1981;84(3):319-35. doi: 10.1007/BF00286022.
The presence of a stable maternal mRNA population in mature oocytes of many species is well established. In this paper we show that the mature egg contains, in addition to these mature mRNAs, a structurally more complex population of RNA transcripts. This latter class of RNA consists of polyadenylated transcripts of repetitive and nonrepetitive DNA elements covalently linked into long interspersed molecules. As much as seventy percent of the polyadenylated egg RNA of Xenopus laevis and Strongylocentrotus purpuratus is represented in this interspersed population. Most of the nonrepetitive DNA sequences represented in the mature mRNA population are also present in the interspersed RNA. These transcripts have an organization similar to that of somatic cell nuclear RNA. Data are presented that suggests some of these interspersed maternal transcripts are unprocessed precursor-like molecules. Some possible functions of this novel class of RNA during early development are discussed.
许多物种的成熟卵母细胞中存在稳定的母体mRNA群体，这一点已得到充分证实。在本文中，我们表明，除了这些成熟的mRNA外，成熟卵子还含有一类结构上更为复杂的RNA转录本群体。后一类RNA由重复和非重复DNA元件的多聚腺苷酸化转录本组成，这些转录本共价连接成长链散布分子。非洲爪蟾和紫球海胆的多聚腺苷酸化卵RNA中，多达70%存在于这种散布群体中。成熟mRNA群体中所代表的大多数非重复DNA序列也存在于散布RNA中。这些转录本的组织方式与体细胞核糖核酸相似。所提供的数据表明，这些散布的母体转录本中的一些是未加工的前体样分子。本文还讨论了这类新型RNA在早期发育过程中的一些可能功能。
