Lee A S, Thomas T L, Lev Z, Britten R J, Davidson E H
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3259-63. doi: 10.1073/pnas.77.6.3259.
This report concerns a set of sea urchin egg and embryo transcripts complementary to a single-copy region of a cloned DNA fragment (Sp88). Three distinct 16-cell embryo polysomal RNA species were found to hybridize with this fragment. These RNAs are about 1700, 3000, and 4000 nucleotides (nt) in length, and the same species were identified in unfertilized eggs. A significant fraction of all three species of the egg and early embryo transcripts is polyadenylylated. At gastrula stage Sp88 transcripts are almost completely confined to the nucleus [Lev, Z., Thomas, T. L., Lee, A. S., Angerer, R. C., Britten, R. J. & Davidson, E. H. (1980) Dev. Biol, 75, in press]. The Sp88 transcripts of gastrulae are present as a fourth RNA species approximately 5800 nt in length. The four species share a sequence element of cloned DNA fragment that is about 1000 nt long. These RNAs constitute a set of alternative partially overlapping transcripts from the same genomic region.
本报告涉及一组与克隆DNA片段（Sp88）的单拷贝区域互补的海胆卵和胚胎转录本。发现三种不同的16细胞胚胎多聚核糖体RNA物种与该片段杂交。这些RNA的长度约为1700、3000和4000个核苷酸（nt），并且在未受精卵中也鉴定出了相同的物种。所有三种卵和早期胚胎转录本的很大一部分都进行了多聚腺苷酸化。在原肠胚阶段，Sp88转录本几乎完全局限于细胞核[列夫，Z.，托马斯，T.L.，李，A.S.，安格勒，R.C.，布里顿，R.J.和戴维森，E.H.（1980年）《发育生物学》，75卷，即将出版]。原肠胚的Sp88转录本以第四种RNA物种的形式存在，长度约为5800 nt。这四种物种共享克隆DNA片段中一个约1000 nt长的序列元件。这些RNA构成了来自同一基因组区域的一组选择性部分重叠转录本。
