Lin Y, Stadler B M, Rabin H
J Biol Chem. 1982 Feb 25;257(4):1587-90.
The poly(A)-containing RNA isolated from a gibbon lymphosarcoma cell line, MLA144, known to release Interleukin 2 (IL2) was microinjected into Xenopus oocytes. The incubation medium from injected oocytes stimulated the DNA synthesis of an IL2-dependent cell culture and also maintained its viability. DNA-stimulating activity appeared in the oocyte incubation medium 6 h after injection and continued to accumulate for at least 96 h. Thymidine incorporation by the IL2-dependent cells is proportional to the concentration of oocyte incubation medium added and the IL2 activity produced by the oocytes is proportional to the amount of poly(A)-RNA injected. Incubation medium of oocytes injected with RNA from another T-cell line, 6G1, which does not produce IL2, did not contain DNA-stimulating activity. The same assay also showed that the IL2 mRNA sedimented at 14-16 S in a nondenaturing sucrose gradient. A series of monoclonal antibodies prepared against human IL2 neutralized the DNA-stimulating activity released by the injected oocytes to the same extent as they neutralized human IL2.
从已知能释放白细胞介素2（IL2）的长臂猿淋巴肉瘤细胞系MLA144中分离出的含聚腺苷酸（poly(A)）的RNA被显微注射到非洲爪蟾卵母细胞中。注射了卵母细胞的孵育培养基刺激了依赖IL2的细胞培养物的DNA合成，并维持了其活力。DNA刺激活性在注射后6小时出现在卵母细胞孵育培养基中，并持续积累至少96小时。依赖IL2的细胞掺入胸苷的量与添加的卵母细胞孵育培养基的浓度成正比，卵母细胞产生的IL2活性与注射的poly(A)-RNA的量成正比。用来自另一个不产生IL2的T细胞系6G1的RNA注射的卵母细胞的孵育培养基不含有DNA刺激活性。相同的测定还表明，IL2 mRNA在非变性蔗糖梯度中沉降在14 - 16 S处。一系列针对人IL2制备的单克隆抗体中和注射的卵母细胞释放的DNA刺激活性的程度与它们中和人IL2的程度相同。
