珠蛋白信使核糖核酸中聚腺苷酸片段的缺失加速了其在非洲爪蟾卵母细胞中的降解。
Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
作者信息
Marbaix G, Huez G, Burny A, Cleuter Y, Hubert E, Leclercq M, Chantrenne H, Soreq H, Nudel U, Littauer U Z
出版信息
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3065-7. doi: 10.1073/pnas.72.8.3065.
Abstract
Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.
摘要
将兔珠蛋白无聚腺苷酸和天然信使核糖核酸制剂显微注射到非洲爪蟾卵母细胞中。通过与放射性互补DNA探针进行分子杂交来测定注射卵母细胞孵育后剩余的珠蛋白信使核糖核酸量，该探针是通过用来自禽成髓细胞瘤病毒的纯化的依赖RNA的DNA聚合酶（逆转录酶）复制珠蛋白信使核糖核酸合成的。结果表明，注射后56小时，约85%的无聚腺苷酸信使核糖核酸分子被降解，而天然含聚腺苷酸的信使核糖核酸链在同一时间段内几乎完全保留。
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Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.珠蛋白信使核糖核酸中聚腺苷酸片段的缺失加速了其在非洲爪蟾卵母细胞中的降解。
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本文引用的文献
1
Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.注射到蛙卵母细胞中的信息稳定性：哺乳动物α和β珠蛋白信息的长寿命
J Mol Biol. 1973 Nov 5;80(3):539-51. doi: 10.1016/0022-2836(73)90421-x.
2
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.米曲霉单链特异性核酸酶的纯化及其他特性
Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.
3
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.无聚腺苷酸兔珠蛋白信使核糖核酸的体外翻译
J Mol Biol. 1974 Sep 5;88(1):233-45. doi: 10.1016/0022-2836(74)90307-6.
4
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.聚腺苷酸片段在非洲爪蟾卵母细胞中珠蛋白信使核糖核酸翻译中的作用。
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5
Translational control of globin synthesis by haemin in Xenopus oocytes.血红素对非洲爪蟾卵母细胞中珠蛋白合成的翻译调控。
Nat New Biol. 1973 Nov 28;246(152):99-102. doi: 10.1038/newbio246099a0.
6
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.注入非洲爪蟾卵母细胞的核糖核酸的降解
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7
Purification of the DNA polymerase of avian myeloblastosis virus.禽成髓细胞瘤病毒DNA聚合酶的纯化
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