Cooper J A, Hunter T
J Cell Biol. 1981 Dec;91(3 Pt 1):878-83. doi: 10.1083/jcb.91.3.878.
We have derived a line of A431 human tumor cells infected with Rous sarcoma virus (RSV). The infected cells contain the RSV-transforming protein, pp60src, which has characteristic tyrosine specific protein kinase activity. As in other RSV-transformed cells, a 36,000-dalton protein is phosphorylated in RSV-infected A431 cells. Addition of epidermal growth factor (EGF) to the cells induces further phosphorylation of this protein. In contrast, this phosphoprotein is not detected in uninfected A431 cells, except when treated with EGF. Increased phosphorylation of the EGF receptor protein and of an 81,000-dalton cellular protein is dependent upon addition of EGF to the culture fluids, in both control and RSV-infected A431 cells. The results are discussed with reference to the similarities and differences between the tyrosine-specific protein kinases induced by RSV and activated by EGF.
我们已获得了一组感染劳氏肉瘤病毒（RSV）的A431人肿瘤细胞系。被感染的细胞含有RSV转化蛋白pp60src，其具有特征性的酪氨酸特异性蛋白激酶活性。与其他RSV转化细胞一样，在RSV感染的A431细胞中，一种36,000道尔顿的蛋白会发生磷酸化。向细胞中添加表皮生长因子（EGF）会诱导该蛋白进一步磷酸化。相比之下，在未感染的A431细胞中未检测到这种磷蛋白，除非用EGF处理。在对照和RSV感染的A431细胞中，EGF受体蛋白和一种81,000道尔顿细胞蛋白的磷酸化增加均依赖于向培养液中添加EGF。文中参照了由RSV诱导和由EGF激活的酪氨酸特异性蛋白激酶之间的异同对结果进行了讨论。
