表皮生长因子与劳氏肉瘤病毒作用之间的异同。
Similarities and differences between the effects of epidermal growth factor and Rous sarcoma virus.
作者信息
Cooper J A, Hunter T
出版信息
J Cell Biol. 1981 Dec;91(3 Pt 1):878-83. doi: 10.1083/jcb.91.3.878.
Abstract
We have derived a line of A431 human tumor cells infected with Rous sarcoma virus (RSV). The infected cells contain the RSV-transforming protein, pp60src, which has characteristic tyrosine specific protein kinase activity. As in other RSV-transformed cells, a 36,000-dalton protein is phosphorylated in RSV-infected A431 cells. Addition of epidermal growth factor (EGF) to the cells induces further phosphorylation of this protein. In contrast, this phosphoprotein is not detected in uninfected A431 cells, except when treated with EGF. Increased phosphorylation of the EGF receptor protein and of an 81,000-dalton cellular protein is dependent upon addition of EGF to the culture fluids, in both control and RSV-infected A431 cells. The results are discussed with reference to the similarities and differences between the tyrosine-specific protein kinases induced by RSV and activated by EGF.
摘要
我们已获得了一组感染劳氏肉瘤病毒（RSV）的A431人肿瘤细胞系。被感染的细胞含有RSV转化蛋白pp60src，其具有特征性的酪氨酸特异性蛋白激酶活性。与其他RSV转化细胞一样，在RSV感染的A431细胞中，一种36,000道尔顿的蛋白会发生磷酸化。向细胞中添加表皮生长因子（EGF）会诱导该蛋白进一步磷酸化。相比之下，在未感染的A431细胞中未检测到这种磷蛋白，除非用EGF处理。在对照和RSV感染的A431细胞中，EGF受体蛋白和一种81,000道尔顿细胞蛋白的磷酸化增加均依赖于向培养液中添加EGF。文中参照了由RSV诱导和由EGF激活的酪氨酸特异性蛋白激酶之间的异同对结果进行了讨论。
相似文献
1
Similarities and differences between the effects of epidermal growth factor and Rous sarcoma virus.表皮生长因子与劳氏肉瘤病毒作用之间的异同。
J Cell Biol. 1981 Dec;91(3 Pt 1):878-83. doi: 10.1083/jcb.91.3.878.
2
Comparison of protein phosphorylations in variant A431 cells with different growth responses to epidermal growth factor.对表皮生长因子具有不同生长反应的A431变异细胞中蛋白质磷酸化的比较。
J Cell Physiol. 1984 Jun;119(3):296-306. doi: 10.1002/jcp.1041190307.
3
Epidermal growth factor receptor metabolism and protein kinase activity in human A431 cells infected with Snyder-Theilen feline sarcoma virus or harvey or Kirsten murine sarcoma virus.感染斯奈德-泰伦猫肉瘤病毒、哈维或柯斯顿鼠肉瘤病毒的人A431细胞中表皮生长因子受体的代谢及蛋白激酶活性
J Virol. 1983 Dec;48(3):752-64. doi: 10.1128/JVI.48.3.752-764.1983.
4
Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells.表皮生长因子可诱导A431人肿瘤细胞中的蛋白质快速发生酪氨酸磷酸化。
Cell. 1981 Jun;24(3):741-52. doi: 10.1016/0092-8674(81)90100-8.
5
Tyrosine phosphorylation of a 50K cellular polypeptide associated with the Rous sarcoma virus transforming protein pp60src.与劳氏肉瘤病毒转化蛋白pp60src相关的一种50K细胞多肽的酪氨酸磷酸化作用。
Mol Cell Biol. 1982 Feb;2(2):199-206. doi: 10.1128/mcb.2.2.199-206.1982.
6
Purified EGF receptor-kinase interacts specifically with antibodies to Rous sarcoma virus transforming protein.纯化的表皮生长因子受体激酶与抗劳斯肉瘤病毒转化蛋白的抗体特异性相互作用。
Nature. 1981 Apr 9;290(5806):516-9. doi: 10.1038/290516a0.
7
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.劳氏肉瘤病毒的转化基因产物使酪氨酸磷酸化。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5. doi: 10.1073/pnas.77.3.1311.
8
Transformation by Rous sarcoma virus: a cellular substrate for transformation-specific protein phosphorylation contains phosphotyrosine.劳氏肉瘤病毒介导的转化：一种用于转化特异性蛋白磷酸化的细胞底物含有磷酸酪氨酸。
Cell. 1980 Oct;21(3):821-8. doi: 10.1016/0092-8674(80)90445-6.
9
Altered epidermal growth factor (EGF)-stimulated protein kinase activity in variant A431 cells with altered growth responses to EGF.在对表皮生长因子（EGF）生长反应改变的A431变异细胞中，EGF刺激的蛋白激酶活性发生改变。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2574-8. doi: 10.1073/pnas.79.8.2574.
10
Phosphorylations of the subcellular matrix in cells transformed by Rous' sarcoma virus.劳氏肉瘤病毒转化细胞中亚细胞基质的磷酸化作用
Eur J Biochem. 1982 Jul;125(2):453-6. doi: 10.1111/j.1432-1033.1982.tb06704.x.
引用本文的文献
1
The Position of EGF Deprivation in the Management of Advanced Non-Small Cell Lung Cancer.表皮生长因子剥夺在晚期非小细胞肺癌治疗中的地位
Front Oncol. 2021 Jun 15;11:639745. doi: 10.3389/fonc.2021.639745. eCollection 2021.
2
The genesis of tyrosine phosphorylation.酪氨酸磷酸化的起源。
Cold Spring Harb Perspect Biol. 2014 May 1;6(5):a020644. doi: 10.1101/cshperspect.a020644.
3
Jeremiah Metzger Lecture. Targeted cancer therapy.耶利米·梅茨格讲座。靶向癌症治疗。
Trans Am Clin Climatol Assoc. 2000;111:95-110; discussion 110-1.
4
Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture.表皮生长因子在无血清细胞培养中抑制A431人表皮样癌的生长。
J Cell Biol. 1982 Apr;93(1):1-4. doi: 10.1083/jcb.93.1.1.
5
Tyrosine phosphorylation of specific proteins after mitogen stimulation of chicken embryo fibroblasts.丝裂原刺激鸡胚成纤维细胞后特定蛋白质的酪氨酸磷酸化
Mol Cell Biol. 1983 Mar;3(3):380-90. doi: 10.1128/mcb.3.3.380-390.1983.
6
Molecular lesions in cancer.癌症中的分子病变
Mol Cell Biochem. 1984;61(2):111-30. doi: 10.1007/BF00222490.
7
Subcellular location of an abundant substrate (p36) for tyrosine-specific protein kinases.酪氨酸特异性蛋白激酶的一种丰富底物（p36）的亚细胞定位。
Mol Cell Biol. 1983 Mar;3(3):340-50. doi: 10.1128/mcb.3.3.340-350.1983.
8
Tumor promoters block tyrosine-specific phosphorylation of the epidermal growth factor receptor.肿瘤启动子可阻断表皮生长因子受体的酪氨酸特异性磷酸化。
Proc Natl Acad Sci U S A. 1984 May;81(10):3034-8. doi: 10.1073/pnas.81.10.3034.
9
The 46,000-dalton tyrosine protein kinase substrate is widespread, whereas the 36,000-dalton substrate is only expressed at high levels in certain rodent tissues.46000道尔顿的酪氨酸蛋白激酶底物分布广泛，而36000道尔顿的底物仅在某些啮齿动物组织中高水平表达。
J Cell Biol. 1984 Feb;98(2):487-97. doi: 10.1083/jcb.98.2.487.
10
Reduced synthesis of pp60src and expression of the transformation-related phenotype in interferon-treated Rous sarcoma virus-transformed rat cells.在干扰素处理的劳氏肉瘤病毒转化的大鼠细胞中，pp60src的合成减少以及与转化相关表型的表达降低。
Mol Cell Biol. 1983 Sep;3(9):1656-64. doi: 10.1128/mcb.3.9.1656-1664.1983.
本文引用的文献
1
Transforming proteins of some feline and avian sarcoma viruses are related structurally and functionally.一些猫和禽肉瘤病毒的转化蛋白在结构和功能上相关。
Cell. 1981 Apr;24(1):145-53. doi: 10.1016/0092-8674(81)90510-9.
2
Evidence that the Abelson virus protein functions in vivo as a protein kinase that phosphorylates tyrosine.有证据表明，阿贝尔森病毒蛋白在体内作为一种使酪氨酸磷酸化的蛋白激酶发挥作用。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1552-6. doi: 10.1073/pnas.78.3.1552.
3
Anti-pp60src antibodies are substrates for EGF-stimulated protein kinase.抗pp60src抗体是表皮生长因子刺激的蛋白激酶的底物。
Nature. 1981 Apr 9;290(5806):519-21. doi: 10.1038/290519a0.
4
Purified EGF receptor-kinase interacts specifically with antibodies to Rous sarcoma virus transforming protein.纯化的表皮生长因子受体激酶与抗劳斯肉瘤病毒转化蛋白的抗体特异性相互作用。
Nature. 1981 Apr 9;290(5806):516-9. doi: 10.1038/290516a0.
5
Regulation of phosphorylation of the beta-subunit of th Ehrlich ascites tumor Na+K+-ATPase by a protein kinase cascade.通过蛋白激酶级联反应对艾氏腹水瘤钠钾ATP酶β亚基磷酸化的调节。
J Biol Chem. 1981 May 10;256(9):4219-27.
6
Tyrosine-specific protein kinase activity associated with p105 of avian sarcoma virus PRCII.与禽肉瘤病毒PRCII的p105相关的酪氨酸特异性蛋白激酶活性。
Virology. 1981 Feb;109(1):223-8. doi: 10.1016/0042-6822(81)90493-1.
7
A strain of Fujinami sarcoma virus which is temperature-sensitive in protein phosphorylation and cellular transformation.一种在蛋白质磷酸化和细胞转化方面对温度敏感的 Fujinami 肉瘤病毒毒株。
Cell. 1980 Dec;22(3):767-75. doi: 10.1016/0092-8674(80)90553-x.
8
Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.与藤浪肉瘤病毒转化基因产物相关的蛋白激酶活性的特性分析
Cell. 1980 Dec;22(3):757-65. doi: 10.1016/0092-8674(80)90552-8.
9
Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity.禽肉瘤病毒Y73的特性：一种独特的转化基因及其产物，一种具有蛋白激酶活性的磷酸化多蛋白。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6199-203. doi: 10.1073/pnas.77.10.6199.
10
The purified product of the transforming gene of avian sarcoma virus phosphorylates tyrosine.禽肉瘤病毒转化基因的纯化产物使酪氨酸磷酸化。
J Biol Chem. 1980 Dec 25;255(24):11973-80.
Zotero 插件