Billings P B, Allen R W, Jensen F C, Hoch S O
J Immunol. 1982 Mar;128(3):1176-80.
systemic lupus erythematosus (SLE) and related rheumatic and connective-tissue diseases are often associated with the production of antibodies directed against a variety of specific cellular components. Recent evidence indicates that two such autoantigens, the Sm and RNP antigens recognized by SLE sera, exist in small ribonucleoprotein complexes found in the nuclei of higher eukaryotes. Studies of the structure and function of these autoantigenic particles with human sera used as probes have been limited because of the multiplicity of autoantibodies often found in an individual serum. Through this communication, we report that MRL/Mp-+/+ (MRL/n) mice, which spontaneously develop a disease exhibiting many of the characteristics of human SLE, possess anti-RNP antibodies in addition to anti-Sm and anti-DNA as previously reported. Spleen cells from one such autoimmune mouse were used to produce a stable hybridoma secreting antibodies that react simultaneously with a protein of Mr 40,000 and a doublet of approximately 70,000, a pattern of reactivity identical to and characteristic of human SLE anti-RNP autoantibodies.
系统性红斑狼疮（SLE）及相关的风湿性和结缔组织疾病常与针对多种特定细胞成分的抗体产生有关。最近的证据表明，SLE血清所识别的两种自身抗原，即Sm和RNP抗原，存在于高等真核生物细胞核中的小核糖核蛋白复合物中。由于个体血清中常发现多种自身抗体，因此以人血清作为探针研究这些自身抗原性颗粒的结构和功能受到了限制。通过本通讯，我们报告MRL/Mp-+/+（MRL/n）小鼠会自发发展出一种表现出许多人类SLE特征的疾病，除了先前报道的抗Sm和抗DNA抗体外，还拥有抗RNP抗体。来自一只这样的自身免疫小鼠的脾细胞被用于产生一种稳定的杂交瘤，该杂交瘤分泌的抗体能同时与40,000道尔顿的蛋白质和大约70,000道尔顿的双峰发生反应，这种反应模式与人类SLE抗RNP自身抗体相同且具有特征性。
