Stocks M R, Williams D G, Maini R N
Clin Exp Immunol. 1987 Mar;67(3):492-9.
The effect of monoclonal autoantibodies on immunoregulation was investigated in MRL/MpJ-lpr/lpr mice. Passive transfer of KSm2 (a monoclonal IgG2a antibody directed against the 16 kD polypeptide of Sm) induced IgG antibodies to the other major immunoreactive polypeptides of Sm (28 and 29 kD) in all mice studied, and to polypeptides of the closely related antigen nRNP/Sm in 63% of the mice. In addition an increment in IgG anti-dsDNA antibodies, and in IgA and IgM anti-Sm antibodies, over control levels was observed. These effects were not due to polyclonal activation since anti-histone antibody levels were unaffected. Two other IgG2a monoclonal antibodies: KSm5 (directed against the 28 and 29 kD Sm polypeptides) and OX 12 (directed against an irrelevant antigen) failed to modulate the autoimmune responses of the mice in any way. These results demonstrate specific antibody-mediated connectivity between B cell clones producing autoantibodies against three distinct antigens.
在MRL/MpJ-lpr/lpr小鼠中研究了单克隆自身抗体对免疫调节的影响。KSm2（一种针对Sm的16 kD多肽的单克隆IgG2a抗体）的被动转移在所有研究的小鼠中诱导产生了针对Sm的其他主要免疫反应性多肽（28 kD和29 kD）的IgG抗体，在63%的小鼠中诱导产生了针对密切相关抗原nRNP/Sm的多肽的IgG抗体。此外，观察到IgG抗双链DNA抗体以及IgA和IgM抗Sm抗体水平高于对照水平。这些效应并非由于多克隆激活，因为抗组蛋白抗体水平未受影响。另外两种IgG2a单克隆抗体：KSm5（针对28 kD和29 kD Sm多肽）和OX 12（针对无关抗原）未能以任何方式调节小鼠的自身免疫反应。这些结果证明了产生针对三种不同抗原的自身抗体的B细胞克隆之间存在特异性抗体介导的联系。
