Bastardo J W, McKimm-Breschkin J L, Sonza S, Mercer L D, Holmes I H
Infect Immun. 1981 Dec;34(3):641-7. doi: 10.1128/iai.34.3.641-647.1981.
Antisera to SA11 virus proteins were prepared by immunizing rabbits with individual polypeptides separated by polyacrylamide gel electrophoresis under reducing or nonreducing conditions; the resulting antisera were characterized by four immunological methods. Results of complement fixation tests with double-shelled rotavirus particles and sera raised against reduced or unreduced proteins of the outer shell of the virus suggested the presence of common antigenic determinants in the outer capsid layers of SA11 and the Northern Ireland strain of calf rotavirus. In this test, antisera to outer shell polypeptides gp34 (O2) and gp25 (O4) cross-reacted with calf rotavirions, whereas those to p62 (O1) and p26 (O3) reacted only with the homologous virus. Antisera to the reduced outer shell proteins of the virus did not neutralize viral infectivity, nor did they possess hemagglutination inhibition activity. Evidence suggesting the presence of type-specific antigenic determinant(s) in the major inner protein p42 (I4) of SA11 virus, capable of inducing neutralizing antibody, is presented and discussed. Antisera produced against unreduced gp34 and p26 polypeptides of the virus contained type-specific neutralizing antibodies. Polypeptide gp34 was also capable of inducing hemagglutination inhibiting antibody. All of the antisera to unreduced polypeptides had agglutinating activity against double-shelled particles of homologous and heterologous rotaviruses.
通过用在还原或非还原条件下经聚丙烯酰胺凝胶电泳分离的单个多肽免疫兔子，制备了针对SA11病毒蛋白的抗血清；通过四种免疫学方法对所得抗血清进行了表征。用双层轮状病毒颗粒以及针对病毒外壳还原或未还原蛋白产生的血清进行补体结合试验的结果表明，SA11和北爱尔兰小牛轮状病毒株的外衣壳层中存在共同的抗原决定簇。在该试验中，针对外壳多肽gp34（O2）和gp25（O4）的抗血清与小牛轮状病毒颗粒发生交叉反应，而针对p62（O1）和p26（O3）的抗血清仅与同源病毒发生反应。针对病毒还原外壳蛋白的抗血清既不能中和病毒感染性，也不具有血凝抑制活性。本文展示并讨论了表明SA11病毒主要内部蛋白p42（I4）中存在能够诱导中和抗体的型特异性抗原决定簇的证据。针对病毒未还原的gp34和p26多肽产生的抗血清含有型特异性中和抗体。多肽gp34也能够诱导血凝抑制抗体。所有针对未还原多肽的抗血清对同源和异源轮状病毒的双层颗粒均具有凝集活性。
