Zissis G, Lambert J P
J Clin Microbiol. 1980 Jan;11(1):1-5. doi: 10.1128/jcm.11.1.1-5.1980.
Five enzyme-linked immunosorbent assay systems were adapted for serotyping human rotavirus strains and were compared with a sensitive complement fixation test in terms of specificity and sensitivity. The assays differed mainly with regard to the antibody systems involved in the double sandwich. Serotype differentiation of 34 rotavirus strains was achieved by determining a neutralization endpoint titer, either with a constant antiserum-varying antigen dilution method or vice versa. The procedure which proved to be highly specific and sensitive was one with two type-specific hyperimmune sera (enzyme-linked immunosorbent assay system 5) instead of only one, as in the four other systems.
五种酶联免疫吸附测定系统被用于对人轮状病毒株进行血清分型，并在特异性和敏感性方面与一种灵敏的补体结合试验进行了比较。这些测定法主要在双夹心所涉及的抗体系统方面有所不同。通过用恒定抗血清-可变抗原稀释法或反之亦然来确定中和终点效价，实现了对34株轮状病毒的血清型区分。被证明具有高度特异性和敏感性的方法是使用两种型特异性超免疫血清（酶联免疫吸附测定系统5），而不像其他四种系统那样只使用一种。
