Mantyh P W
J Comp Neurol. 1982 Feb 1;204(4):349-63. doi: 10.1002/cne.902040406.
Anatomical staining methods including Nissl, Weil, Golgi, and horseradish peroxidase stain have been used to elucidate the cyto- and myeloarchitectural organization of the periaqueductal gray in monkey, cat, and rat. From these various staining methods it appears that the periaqueductal gray is composed of a tightly packed group of cells, which show a slight increase in soma size, dendritic diameter, and degree of myelinization from central to peripheral borders. This central gray region contains a wide variety of cell types including multipolar, fusiform, stellate, and pyramidal neurons. Clearly delineated subnuclei, distinguished on the basis of soma size, dendritic arborizations, pigmentation, or evidence of cytological individuality could not be discerned in this study. Together with the immunohistochemical and connectivity studies the present data suggest that the neuronal organization of the PAG could be described as a mosaic of clusters of functional related neurons rather than as three distinct subnuclei, each with its own unique cytoarchitecture and connectivity.
包括尼氏染色、魏尔染色、高尔基染色和辣根过氧化物酶染色在内的解剖学染色方法已被用于阐明猴、猫和大鼠中脑导水管周围灰质的细胞结构和髓鞘结构组织。从这些不同的染色方法来看，中脑导水管周围灰质由一组紧密排列的细胞组成，从中央边界到外周边界，这些细胞的胞体大小、树突直径和髓鞘化程度略有增加。这个中央灰质区域包含多种细胞类型，包括多极神经元、梭形神经元、星形神经元和锥体细胞。在本研究中，未能辨别出基于胞体大小、树突分支、色素沉着或细胞学个体性证据而区分的清晰界定的亚核。结合免疫组织化学和连接性研究，目前的数据表明，中脑导水管周围灰质的神经元组织可被描述为功能相关神经元簇的镶嵌体，而不是三个不同的亚核，每个亚核都有其独特的细胞结构和连接性。
