Veit B C, Hamilton T A, Kotb M
J Immunol. 1982 Apr;128(4):1700-4.
Rabbit antiserum to rat peritoneal exudate (PE) macrophage (M phi) antigens was prepared and its reactivity with cell surface proteins of M phi, granulocytes, and lymphocytes was studied by one- and two-dimensional polyacrylamide gel electrophoresis (PAGE). A total of 14 membrane antigens were identified of which three were found to be expressed only by M phi and granulocytes. By one-dimensional analysis, a protein with an approximate m.w. of 105,000 was present on splenic and PE M phi and on splenic lymphocytes. Two-dimensional analysis revealed that this band was heterogeneous and contained at least three species, one of which was restricted to expression on M phi and granulocytes. A second protein of 150,000 daltons was resolved into two species by two-dimensional analysis. Both of these species were present on M phi and granulocytes but not on lymphocytes. Both the 105,000- and 150,000-dalton proteins were glycosylated. Because the 105,000- and 150-000-dalton proteins expressed by M phi were also expressed by granulocytes, is is likely that these are differentiation antigens whose expression is a characteristic property of cells within both monocytoid and myeloid lineages. All three 105,000-dalton species and one of the two 150,000-dalton species were detected on mouse M phi, indicating their expression is not unique to the rat.
制备了兔抗大鼠腹腔渗出液（PE）巨噬细胞（M phi）抗原的抗血清，并通过一维及二维聚丙烯酰胺凝胶电泳（PAGE）研究了其与M phi、粒细胞和淋巴细胞细胞表面蛋白的反应性。共鉴定出14种膜抗原，其中3种仅在M phi和粒细胞中表达。通过一维分析，在脾脏和PE M phi以及脾脏淋巴细胞上存在一种分子量约为105,000的蛋白质。二维分析显示该条带具有异质性，包含至少三种成分，其中一种仅在M phi和粒细胞上表达。二维分析将第二种150,000道尔顿的蛋白质解析为两种成分。这两种成分均存在于M phi和粒细胞上，但不存在于淋巴细胞上。105,000道尔顿和150,000道尔顿的蛋白质均被糖基化。由于M phi表达的105,000道尔顿和15,000道尔顿的蛋白质也在粒细胞中表达，因此这些可能是分化抗原，其表达是单核细胞系和髓细胞系内细胞的特征性属性。在小鼠M phi上检测到了所有三种105,000道尔顿的成分以及两种150,000道尔顿成分中的一种，表明它们的表达并非大鼠所特有。
