Rumpold H, Förster O, Böck G, Swetly P, Riedl M
Immunology. 1982 Apr;45(4):637-43.
Hybridoma antibodies were produced by fusing spleen cells from mice immunized with rat alveolar macrophages (AM) with P3 x 63Ag8/653 myeloma cells. Some of the hybridomas were processed to achieve monoclonality. Reactivity was tested in an indirect membrane immunofluorescence assay. One of the monoclonal antibodies, called VEP6 antibody, reacted with rat AM but not with peritoneal or splenic macrophages and not with peripheral blood monocytes of this species. It was also unreactive with cells from thymus, bone marrow, non-adherent spleen cells, granulocytes and erythrocytes, but it reacted with thrombocytes. Corynebacterium parvum activated AM were slightly less reactive then 'resident' AM, as determined in a fluorescence activated cell sorter. The VEP6 antigen was studied in polyacrylamide gel electrophoresis after immunoprecipitation from 125I-surface-labelled-macrophage NP40-lysates. Four specific radioactive bands were seen in the precipitates corresponding to molecular weights of 31, 33, 35 and 37,000. A slurred band was seen in this molecular weight range when immunoprecipitates of surface labelled thrombocytes were analysed. This study confirms the heterogeneity of macrophages by showing that certain antigens may be expressed only on a subpopulation of macrophages.
用大鼠肺泡巨噬细胞（AM）免疫的小鼠脾细胞与P3 x 63Ag8/653骨髓瘤细胞融合，产生杂交瘤抗体。对其中一些杂交瘤进行处理以获得单克隆性。在间接膜免疫荧光试验中检测反应性。其中一种单克隆抗体，称为VEP6抗体，与大鼠AM反应，但不与该物种的腹膜或脾巨噬细胞以及外周血单核细胞反应。它也不与来自胸腺、骨髓、非贴壁脾细胞、粒细胞和红细胞的细胞反应，但与血小板反应。在荧光激活细胞分选仪中测定，短小棒状杆菌激活的AM的反应性略低于“驻留”AM。从125I表面标记的巨噬细胞NP40裂解物中进行免疫沉淀后，在聚丙烯酰胺凝胶电泳中研究VEP6抗原。在沉淀物中可见四条特异性放射性条带，对应分子量为31、33、35和37000。分析表面标记血小板的免疫沉淀物时，在该分子量范围内可见一条模糊条带。这项研究通过表明某些抗原可能仅在巨噬细胞亚群上表达，证实了巨噬细胞的异质性。
