使用Cytodex清除巨噬细胞。
Macrophage depletion using Cytodex.
作者信息
Ren E C
出版信息
J Immunol Methods. 1982;49(1):105-11. doi: 10.1016/0022-1759(82)90371-4.
Cytodex (microcarrier) beads were adapted for use as an adhering surface to deplete macrophages from a mouse spleen cell population. Non-adherent cells recovered from the incubation of one mouse spleen with 50 mg of Cytodex showed good viability and had markedly reduced proliferative response to the T cell mitogens phytohemagglutinin and concanavalin A. Response to lipopolysaccharide was, however, less clearly affected. Peritoneal exudate cells, and adherent cells recovered from the Cytodex by trypsinization, were able to restore the mitogenic response of non-adherent cells.
Cytodex（微载体）珠被用作黏附表面，以从小鼠脾细胞群体中去除巨噬细胞。用50毫克Cytodex孵育一只小鼠脾脏后回收的非黏附细胞显示出良好的活力，并且对T细胞有丝分裂原植物血凝素和刀豆球蛋白A的增殖反应明显降低。然而，对脂多糖的反应受影响不太明显。腹腔渗出细胞以及通过胰蛋白酶消化从Cytodex回收的黏附细胞能够恢复非黏附细胞的促有丝分裂反应。
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