Ohtani A, Kumazawa Y, Fujisawa H, Nishimura C
J Reticuloendothel Soc. 1982 Sep;32(3):189-200.
The inhibitory action of 2-chloroadenosine (2-CA) on murine macrophages was investigated by means of concanavalin A (Con A)-induced T-cell proliferative response and of in vitro antibody response to sheep red blood cells (SRBC). The viability of peritoneal exudate cells (PEC) markedly decreased when treated with 0.1 mM 2-CA at 37 degrees C for 2 hr before cell cultures. Morphological changes were also observed in PEC treated with 2-CA. When spleen cells were treated with 2-CA or macrophages were depleted from the spleen cells, Con A-induced proliferative responses were markedly reduced unless normal spleen adherent cells (SAC) were added back to the cultures. However, reduced response could not be restored to the control level by the addition of 2-CA-treated SAC. AntiSRBC antibody response was also suppressed by pretreatment of spleen cells with 2-CA when adenosine was not present during the period of the treatment. Therefore, 2-CA is a useful cytotoxic agent for inhibiting the accessory functions of macrophages.
通过伴刀豆球蛋白A（Con A）诱导的T细胞增殖反应以及对绵羊红细胞（SRBC）的体外抗体反应，研究了2-氯腺苷（2-CA）对小鼠巨噬细胞的抑制作用。在细胞培养前，将腹腔渗出细胞（PEC）在37℃用0.1 mM 2-CA处理2小时，其活力显著下降。在用2-CA处理的PEC中也观察到形态学变化。当用2-CA处理脾细胞或从脾细胞中去除巨噬细胞时，Con A诱导的增殖反应显著降低，除非将正常脾黏附细胞（SAC）重新添加到培养物中。然而，添加经2-CA处理的SAC不能将降低的反应恢复到对照水平。当在处理期间不存在腺苷时，用2-CA预处理脾细胞也会抑制抗SRBC抗体反应。因此，2-CA是一种用于抑制巨噬细胞辅助功能的有用细胞毒性剂。
